文献类型：期刊文献

英文题名：A dual pH and temperature responsive polymeric fluorescent sensor and its imaging application in living cells

作者：Yin, Liyan[1];He, Chunsheng[1];Huang, Chusen[1];Zhu, Weiping[1];Wang, Xin[1];Xu, Yufang[1];Qian, Xuhong[1]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：48

期号：37

起止页码：4486

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000302559100029)】；

基金：The authors are grateful for the financial support from the National Natural Science Foundation of China (21076077), the National Science and Technology Pillar Program of China (2011BAE06B02), the National Basic Research Program of China (2010CB126100), the National High Technology Research and Development Program of China (2011AA10A207, 2012AA061601), the Fundamental Research Funds for the Central Universities and the Shanghai Leading Academic Discipline Project (B507). We also wish to thank Mrs Qin Yin for her assistance with confocal fluorescence images.

语种：英文

摘要：A polymeric fluorescent sensor PNME, consisting of A4 and N-isopropylacrylamide (NIPAM) units, was synthesized. PNME exhibited dual responses to pH and temperature, and could be used as an intracellular pH sensor for lysosomes imaging. Moreover, it also could sense different temperature change in living cells at 25 and 37 degrees C, respectively.