文献类型：期刊文献

英文题名：Oxygen-Carrying and Antibacterial Fluorinated Nano-Hydroxyapatite Incorporated Hydrogels for Enhanced Bone Regeneration

作者：Huang, Baoxuan[1];Chen, Mingjiao[2];Tian, Jia[1];Zhang, Yuanhao[1];Dai, Zhaobo[1];Li, Jin[2];Zhang, Weian[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Meilong Rd 130, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Med, Shanghai Key Lab Orbital Dis & Ocular Oncol, Dept Ophthalmol,Peoples Hosp 9, Zhizaoju Rd 639, Shanghai 200011, Peoples R China

年份：2022

卷号：11

期号：12

外文期刊名：ADVANCED HEALTHCARE MATERIALS

收录：;EI(收录号：20221411882239);WOS:【SCI-EXPANDED(收录号:WOS:000776478700001)】；

基金：B.H. and M.C. contributed equally to this work. All animal procedures were done in accordance with the Guidelines for Care and Use of Laboratory Animals of the Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine and experiments were approved by the Animal Ethics Committee of the Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine (SYXK(Shanghai) 2016-0016).This work was financially supported by the National Natural Science Foundation of China (21875063, 22075079, and 81870688), the Science and Technology Commission of Shanghai Municipality for the Shanghai International Cooperation Program (19440710600).

语种：英文

外文关键词：antibacterials; bone regeneration; hydrogels; oxygen-carrying

摘要：Insufficient oxygen availability in tissue engineering is one of the major factors for the failure of clinical transplantation. One potential strategy to conquer this limitation is the fabrication of spontaneous and continuous oxygen supplying scaffolds for in situ tissue regeneration. In this work, a versatile fluorine-incorporating hydrogel is designed which can not only timely and continuously supply oxygen for mesenchymal stem cells (MSCs) to overcome deficient oxygen before vascularization in scaffolds, but can present a higher antibacterial capability to avoid bacterial infections. The HAp@PDA-F nanoparticles are first prepared and then incorporated with the quaternized and methacrylated chitosan forming CS/HAp@PDA-F by photo-crosslinking. In vitro results indicate that CS/HAp@PDA-F hydrogel has outstanding mechanical performance, moreover, it also has the oxygen-carrying ability to prolong survival ability, enhance proliferation activity, and preserve osteogenic differentiation potency and promote osteogenic-related genes expression of rat bone mesenchymal stem cells (rBMSCs) under hypoxic environment. Furthermore, the CS/HAp@PDA-F hydrogel can inhibit the growth of Staphylococcus aureus and Escherichia coli, providing a good antibacterial activity. Additionally, in vivo experiments demonstrate higher bone volume and bone mineral density, and more new bone tissue generation in CS/HAp@PDA-F group than in CS/HAp@PDA group. These results indicate that the rational design of fluorinated hydrogel possesses a good clinical application prospect for bone regeneration.