文献类型：专利

英文题名：New polypeptide having programmed cell death protein 1 (PD-1) binding activity, useful for e.g. inhibiting binding of PD-1 to PD-ligand 1, and treating e.g. bacterial, viral or fungal infections

作者：LI H;LIU X;ZHU L;LI Q;QUAN L;WU F;ZHAO Z

机构：[1]UNIV EAST CHINA SCI & TECHNOLOGY

申请号：WO2017162208-A1

申请日：2017-03-24

公开日：2017-09-28

语种：英文

收录：DERWENT

摘要：NOVELTY - A polypeptide (I) having a programmed cell death protein 1 (PD-1) binding activity, is new, where the polypeptide has length of 15-18 amino acids. USE - The polypeptide is useful in pharmaceutical composition for preventing tumor, and treating bacterial, viral or fungal infections and inflammatory diseases. The tumor is chosen from melanoma, lung cancer (preferably non-small cell lung cancer), kidney cancer, ovarian cancer, prostate cancer, breast cancer, colon cancer, bone cancer, pancreatic cancer, skin cancer, head and neck cancer, uterine cancer, rectal cancer, anal cancer, gastric cancer, testicular cancer, fallopian tube cancer, endometrial cancer, cervical cancer, vaginal cancer, vulvar cancer, Hodgkin's disease, non-Hodgkin's lymphoma, esophageal cancer, small bowel cancer, endocrine system cancer, thyroid cancer, parathyroid cancer, adrenal cancer, soft tissue sarcoma, urinary tract cancer, penile cancer, acute myeloid leukemia, chronic myeloid leukemia, acute lymphoblastic leukemia, chronic lymphocytic leukemia, pediatric solid tumors, lymphocytic lymphoma, bladder cancer, kidney or ureteral cancer, renal pelvis cancer, CNS tumors, primary CNS lymphoma, tumor angiogenesis, spinal axis tumors, brain stem glioma, pituitary adenoma, kaposi sarcoma, epidermoid carcinoma, squamous cell carcinoma and T-cell lymphoma. The viral infections are caused by hepatitis virus (type A, type B and type C), sporadic virus, influenza virus, adenovirus, coronavirus, measles virus, dengue virus, poliovirus or rabies virus. The bacterial infections are chosen from Chlamydia, Rickettsia, Mycobacterium, Staphylococcus, Streptococcus pneumoniae, cholera and tetanus. The fungal infection is caused by Candida or Aspergillus. The inflammatory disease is chosen from ankylosing spondylitis, autoimmune hemolytic anemia, arthritis, myasthenia gravis, systemic lupus erythematosus, rheumatoid arthritis, pernicious anemia and polymyositis (all claimed). ADVANTAGE - The polypeptide has high purity, reduced molecular weight, improved tissue affinity, high specificity, less immunogenicity, enhanced tissue permeability and increased affinity towards PD-1 protein. The polypeptide is prepared in a cost-effective manner. DETAILED DESCRIPTION - A polypeptide of formula: X0-X1-Asp-Tyr-X2-Arg-X3-Tyr-X4 (I) having a programmed cell death protein 1 (PD-1) binding activity, is new, where the polypeptide has length of 15-18 amino acids. X0=Gly-Asn, Thr-Glu or Phe-Asn, or absent; X1=Trp, Lys or Met; X2= Asn-Ser-Gln, Arg-His-Gly-Asn-Ile, Ser-Leu-Glu-Glu-Leu, Ser-Trp-Lys-Ser-Glu-Gln-Asn, Cys-Pro-Cys, Cys-His-Gly-Pro-Cys, Cys-Ala-Glu-Pro-Leu, or Ser-Trp-Cys-Pro-Cys; X3= Ala-Gln-Leu, Met-Lys-Leu-Ala, Glu-Lys-Ala-Lys, Leu-Lys-Glu-Ala, or Glu-Lys-Cys-Lys;and X4= Asn-Gln, Asp-Leu, Lys, Asp-Leu or Arg. INDEPENDENT CLAIMS are included for the following: (1) an isolated nucleic acid molecule encoding the polypeptide; and (2) a host cell comprising the isolated nucleic acid molecule.