文献类型：期刊文献

中文题名：基于微流控的海藻酸钙空心纤维的制备及其在细胞培养中的应用

英文题名：Fabrication of Calcium-alginate Hydrogel Hollow Fibers Based on Microfluidic Method for Cell Encapsulation and Culture

作者：郑焯元[1];朱成成[1];吴松挺[1];岑莲[1];赵世成[1];周帅[1]

机构：[1]华东理工大学上海市多相结构材料化学工程重点实验室,产品工程系,上海200237

年份：2018

卷号：32

期号：A02

起止页码：168

中文期刊名：材料导报

外文期刊名：Materials Reports

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金(21476085;21606084)。

语种：中文

中文关键词：微流控;空心纤维;海藻酸钙;细胞培养

外文关键词：hollow fiber;microfluidic;calcium-alginate;cell culture

摘要：海藻酸钙水凝胶空心纤维在药物传递、组织工程等领域具有潜在的应用价值。但传统的纤维制备方法,如静电纺丝或模具法等,很难或无法制备海藻酸钙空心纤维。本研究首先采用自制的两相微流控装置,连续制备出形貌均一稳定的海藻酸钙空心纤维,并通过显微镜和扫描电子显微镜(SEM)对其空心结构进行了表征。然后考察了装置中内相毛细管尖端管径、内外相流体流速比以及氯化钙浓度对空心纤维内外径尺寸和厚度的影响,结果表明,空心纤维的尺寸在一定范围内可以进行调控。最后将这种空心纤维应用于封装和培养L929成纤维细胞,结果表明,成纤维细胞存活率较高,并且有沿着空心纤维内壁成直线生长的趋势。这种方法为纤维状细胞的培养提供了一种思路。
Calcium-alginate hydrogel hollow microfibers have a potentiality to be utilized in many fields such as drug delivery and tissue engineering.However,it is difficult to be fabricated by the conventional methods,such as electrospinning and molding.Here,a one-step,continuous and controllable process to fabricate calcium-alginate hydrogel hollow microfibers using capillary microfluidics was presented.The hollow structure was demonstrated directly by scanning electron microscope(SEM)and optical microscope.The diameter of hollow fibers could be flexibly adjusted by changing the diameter of orifice within the device and core and sheath flow rates and concentrations of CaCl2 with certain range.L929 fiberoblast type was chosed as a model system and was encapsulated in the hollow fiber using the mentioned method with high viability and the trend of linear cell location.This simple method of cell culture provides a promising way that can assemble fiber-shaped cellular structure in vivo.