文献类型：期刊文献

英文题名：Biochemical properties and potential applications of an organic solvent-tolerant lipase isolated from Serratia marcescens ECU1010

作者：Zhao, Li-Li[1];Xu, Jian-He[1];Zhao, Jian[1];Pan, Jiang[1];Wang, Zhi-Long[2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biocatalysis & Bioproc, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China

年份：2008

卷号：43

期号：6

起止页码：626

外文期刊名：PROCESS BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000256569600005)】；

语种：英文

外文关键词：lipase; Serratia marcescens; organic solvent tolerance; enantiomeric resolution; chiral compounds; biochemical properties

摘要：An extracellular lipase was purified to homogeneity with a purification factor of 5.5-fold from a bacterial strain Serratia marcescens ECU1010. The purified lipase is a dimer with two homologous subunits, of which the molecular mass is 65 kDa, and the pI is 4.2. The pH and temperature optima were shown to be pH 8.0 and 45 degrees C, respectively. Among p-nitrophenyl esters of fatty acids with varied chain length, the lipase showed the maximum activity on p-nitrophenyl myristate (C(14)). The lipase was activated by some surfactants such as Gum Arabic, polyvinyl alcohol (PVA) and Pg350me, but not by Ca(2+). The enzyme displayed pretty high stability in many water miscible and immiscible solvents. This is a unique property of the enzyme which makes it extremely suitable for chemo-enzymatic applications in non-aqueous phase organic synthesis including enantiomeric resolution. Several typical chiral compounds were tested for kinetic resolution with this lipase, consequently giving excellent enantioselectivities (E = 83 similar to> 100) for glycidyl butyrate (GB), 4-hydroxy-3-methyl-2-(2-propenyl)-2-cyclopenten-1-one acetate (HMPCA), naproxen methyl ester (NME) and trans-3-(4'-methoxyphenyl) glycidic acid methyl ester (MPGM). (C) 2008 Elsevier Ltd. All rights reserved.