文献类型：期刊文献

英文题名：Fabrication and Characterization of Collagen/PVA Dual-Layer Membranes for Periodontal Bone Regeneration

作者：Zhou, Tian[1,2];Chen, Siwei[3];Ding, Xinxin[2,4];Hu, Zhihuan[3];Cen, Lian[3];Zhang, Xiaomeng[2,4]

机构：[1]Shanghai Jiao Tong Univ, Coll Stomatol, Shanghai Peoples Hosp 9, Dept Oral Maxillofacial Head & Neck Oncol,Sch Med, Shanghai, Peoples R China;[2]Shanghai Res Inst Stomatol, Shanghai Key Lab Stomatol, Natl Clin Res Ctr Oral Dis, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, Dept Prod Engn, Shanghai Key Lab Multiphase Mat Chem Engn, State Key Lab Chem Engn,Sch Chem Engn, Shanghai, Peoples R China;[4]Shanghai Jiao Tong Univ, Coll Stomatol, Shanghai Peoples Hosp 9, Dept Implant Dent,Sch Med, Shanghai, Peoples R China

年份：2021

卷号：9

外文期刊名：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY

收录：;EI(收录号：20212610552928);WOS:【SCI-EXPANDED(收录号:WOS:000664507700001)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 81801022).

语种：英文

外文关键词：dual-layer; periodontal; collagen; PVA; GTR

摘要：Guided tissue regeneration (GTR) is a promising treatment for periodontal tissue defects, which generally uses a membrane to build a mechanical barrier from the gingival epithelium and hold space for the periodontal regeneration especially the tooth-supporting bone. However, existing membranes possess insufficient mechanical properties and limited bioactivity for periodontal bone regenerate. Herein, fish collagen and polyvinyl alcohol (Col/PVA) dual-layer membrane were developed via a combined freezing/thawing and layer coating method. This dual-layer membrane had a clear but contact boundary line between collagen and PVA layers, which were both hydrophilic. The dual membrane had an elongation at break of 193 +/- 27% and would undergo an in vitro degradation duration of more than 17 days. Further cell experiments showed that compared with the PVA layer, the collagen layer not only presented good cytocompatibility with rat bone marrow-derived mesenchymal stem cells (BMSCs), but also promoted the osteogenic genes (RUNX2, ALP, OCN, and COL1) and protein (ALP) expression of BMSCs. Hence, the currently developed dual-layer membranes could be used as a stable barrier with a stable degradation rate and selectively favor the bone tissue to repopulate the periodontal defect. The membranes could meet the challenges encountered by GTR for superior defect repair, demonstrating great potential in clinical applications.