文献类型：期刊文献

中文题名：酶响应型菊酯/二氧化硅纳米粒的制备及性质

英文题名：Preparation and Characterization of Enzyme-Responsive LC/HMS Nanoparticles

作者：余圣[1];方夏伦[1];邹爱华[1]

机构：[1]华东理工大学化学与分子工程学院,上海200237

年份：2021

卷号：47

期号：5

起止页码：547

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD_E2021_2022】；

基金：国家自然科学基金(21872051);国家重点研发计划(2017YFD0200306)。

语种：中文

中文关键词：控释;酶响应型;纳米农药;介孔二氧化硅;高效氯氟氰菊酯

外文关键词：controlled release;enzyme responsiveness;nano-pesticide;mesoporous silica;lambda-cyhalothrin

摘要：以高效氯氟氰菊酯(LC)为模型药物,通过物理吸附作用将LC负载在氨基化中空介孔二氧化硅(HMSN)纳米粒中,通过酰胺化反应在HMSN表面接枝羧甲基-β-环糊精(CM-β-CD)作为封堵分子,制备得到α-淀粉酶响应型CM-β-CD/LC/HMSN纳米粒。通过傅里叶变换红外光谱(FT-IR)、N2物理吸附-脱附及Zeta电位测试等实验证实该纳米粒制备的可行性。体外释放实验结果表明,在α-淀粉酶存在的条件下,CM-β-CD/LC/HMSN中LC的释放率可达55%,在无酶条件下LC的释放率仅约为20%,表明该农药配方的酶依赖性较强。分别采用浸叶法和浸虫法测定CM-β-CD/LC/HMSN对黏虫幼虫的杀虫活性,浸叶法测定结果表明CM-β-CD/LC/HMSN对黏虫幼虫具有良好的杀虫效果,但浸虫法效果较差。这一差异的原因可能是CM-β-CD/LC/HMSN在靶标幼虫体内可酶解环糊精从而释放LC,导致黏虫幼虫死亡。
Smart nano-pesticides have been prepared to efficiently deliver a sufficient amount of active ingredients in response to different stimuli,employing targeted and controlled release mechanisms.In this paper,an enzymeresponsive carrier for controlled release of lambda-cyhalothrin(LC)was prepared through loading LC into aminated hollow mesoporous silica(HMSN)nanoparticles by physical adsorption,and then carboxymethyl-β-cyclodextrin(CM-β-CD)was coated onto the surface of HMSN using amidation reaction as the blocking molecule.A series of physicochemical characterizations showed the successful construction of the CM-β-CD/LC/HMSN material.An in vitro release test showed that the LC release amount from CM-β-CD/LC/HMSN reached up to 55%in the presence ofα-amylase,while that was only about 20%in the absence of the enzyme,suggesting that the pesticide release was dependent on enzymatic activity.Biological tests showed that the pesticide formulation had good insecticidal effect on Mythimna separata larvae.Based on the results obtained by different experimental methods,we believe that the nanoparticles would be useful for releasing LC after degradation of cyclodextrin enzymatically in vivo,suppressing the survival of Mythimna separata larvae.