文献类型：期刊文献

英文题名：Formaldehyde inhibits development of T lymphocytes in mice

作者：Ma, Huijuan[1,2];Song, Xiaodong[3];Zhang, Weiwei[1,2];Ling, Xinyue[1,2];Yang, Xiaoning[1,2];Wu, Wenwei[1,2];Lou, Kaiyan[1,2];Xu, Huan[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Dept Pharmaceut Sci, Shanghai, Peoples R China;[3]Fudan Univ, Hua Shan Hosp North, Med Lab Dept, Shanghai, Peoples R China

年份：2020

卷号：102

期号：9

起止页码：473

外文期刊名：TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY

收录：;EI(收录号：20203709157855);WOS:【SCI-EXPANDED(收录号:WOS:000583154600004)】；

基金：This work was funded by National Natural Science Foundation of China [grant No. 21906057 to H.X., grant No.21577037 to K.L.] and Shanghai Science and Technology Committee through the Shanghai Sailing Program [grant No. 19YF1412500 to H.X.].

语种：英文

外文关键词：Formaldehyde; selective inhibition; CD8+T cells; T cell development; Runx3; ThPOK

摘要：Formaldehyde (FA) is a ubiquitous environmental pollutant and a group 1 carcinogen. CD8+ T cells are specific tumor killers in the adaptive immune system. In this study, 2.0 mg/m(3) respiratory FA exposure to male mice was found to selectively reduce mature CD8+ T cells in the spleen and the CD8 T cells in the thymus. In order to monitor the exposure of FA in vitro, a fluorescent FA donor was utilized in the treatment of murine thymocytes. Cytotoxicity induced by FA was found to be not selective at single positive stage. Significant inhibition of the runt-related transcription factor-3, the critical transcriptional factors for CD8 lineage commitment, was found in FA-treated double positive cells. ThPOK, the transcriptional factor for CD4 commitment, was stimulated by FA in double positive cells. Increased expression of special AT-rich sequence-binding protein-1 and decreased expression of B-cell lymphoma/leukemia 11B, which are the upstream regulators of ThPOK and the runt-related transcription factor-3, were also observed in FA-treated double positive cells. These results demonstrate that FA could selectively inhibit the development of CD8+ T cells via the alteration of transcriptional factor expressions of thymocytes at double positive stages, which would potentially interfere with the immune defense against cancers.