详细信息
文献类型:期刊文献
中文题名:利用固定化酵母细胞生产ATP
英文题名:Biosynthesis of ATP by using Immobilized Beer Yeast Cells
作者:邱蔚然[1];徐茜[1];张玉岭[1];许娜飞[1]
机构:[1]华东化工学院生化工程研究所
年份:1991
卷号:17
期号:4
起止页码:421
中文期刊名:华东化工学院学报
收录:国家哲学社会科学学术期刊数据库;Scopus;CSCD:【CSCD2011_2012】;
语种:中文
中文关键词:真菌;固定化;细胞;三磷酸腺苷
外文关键词:adenosine triphosphate; immobilized cells; immobilized beer yeast cells; yeast cells; getatin-glutaraldehyde
摘要:利用明胶戊二醛制备的固定化啤酒酵母细胞,由腺苷酸(AMP)生产腺嘌呤核苷三磷酸(ATP)。在含有15 mmol/L AMP,75 mmol/L葡萄糖,5mmol/L硫酸镁(MgSO_4·7 H_2O)的0.2 mol/L磷酸缓冲液(pH 7.0)中,在34℃振荡反应1h,ATP转化率可达100%,分批连续反应10次,ATP转化率保持在90%以上。
The immoblized beer yeast cells entrapped in gelatinglutaraldehyde were used to biosynthesize ATP from AMP. Results of the experiments indicate that in a 0.2 mmol/L potassium phosphate buffer, pH 7.0, containing 75 mmol/L glucose, 15 mmol/L AMP and 5 mmol/L MgSO_4. 7H_2O, the conversion rate of ATP can reach 100% at 34℃ within 1 h. For 10 consecutive batches, the conversion rate of ATP can be maintained over 90%.
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