文献类型：期刊文献

英文题名：Harnessing Gluconobacter oxydans DSM2003 for enantioselective diol oxidation: Substrate scope, stereochemical control, and bioreactor application

作者：Gao, Qiong[1,3];Yuan, Kui[2];Wei, Dongzhi[1];Wu, Xinyan[2]

机构：[1]East China Univ Sci & Technol, Inst Biochem, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China;[3]Shanghai Seezymes Biotechnol Co Ltd, Shanghai, Peoples R China

年份：2026

卷号：200

外文期刊名：ENZYME AND MICROBIAL TECHNOLOGY

收录：;EI(收录号：20262120769791);WOS:【SCI-EXPANDED(收录号:WOS:001783162700001)】；

基金：This research was financially supported by the New Century Talent Project grant No.2002CCA400. The authors gratefully acknowledge the technical assistance of the analytical laboratory of ECUST.

语种：英文

外文关键词：Biocatalysis; Gluconobacter oxydans; Alcohol oxidation; Enantioselective synthesis; Green chemistry; Mandelic acids; omega-Hydroxy carboxylic acids

摘要：The substrate specificity and synthetic applicability of Gluconobacter oxydans DSM2003 were systematically examined. G. oxydans DSM2003 catalyzed the oxidation of both aromatic and aliphatic primary alcohols to the corresponding carboxylic acids in high yields. The catalytic process proceeded through sequential action of membrane-bound alcohol dehydrogenase (mADH), which is pyrroloquinoline quinone (PQQ)-dependent, and membrane-bound aldehyde dehydrogenase (mALDH), which is molybdopterin-dependent; both enzymes are membrane-bound with their active sites facing the periplasmic space. Electrons are transferred via the ubiquinone pool to a terminal oxidase, driving the incomplete but highly selective oxidation reactions. Furthermore, stereoselective oxidation of phenyl 1,2-ethanediols to (R)-mandelic acids with excellent enantiomeric excess (up to 95% e.e.) and selective oxidation of alpha,omega-diols with four or fewer methylene groups to omega-hydroxy carboxylic acids were achieved. This biocatalytic approach offered mild reaction conditions, excellent enantioselectivity, and environmentally friendly processing, making it highly attractive for sustainable synthesis.