文献类型：期刊文献

英文题名：Comprehensive HILIC x RPLC with mass spectrometry detection for the analysis of saponins in Panax notoginseng

作者：Xing, Qianqian[1];Liang, Tu[1];Shen, Guobin[1];Wang, Xinliang[1];Jin, Yu[1];Liang, Xinmiao[1]

机构：[1]E China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Sch Pharm, Shanghai 200237, Peoples R China

年份：2012

卷号：137

期号：9

起止页码：2239

外文期刊名：ANALYST

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000302308600040)】；

基金：This work was supported by the National Natural Science Funds for Distinguished Young Scholars (20825518) and the National Natural Science Funds (21005028).

语种：英文

摘要：A comprehensive off-line two-dimensional liquid chromatography (2D-LC) method coupling hydrophilic interaction liquid chromatography (HILIC) and reversed phase liquid chromatography (RPLC) was developed in this study to detect as many saponins as possible in extracts of Panax notoginseng. The orthogonality of the 2D HILIC x RPLC was up to 81%, and the peak capacity was 10200. In total, 224 saponins were found, and some of them were trace amounts. Besides, a screening table designed by adding molecular weights of possible aglycones and sugars was constructed to help rapidly characterize the saponins using MS information. Unfortunately, the structure of saponins could not be identified by using only MS information.