文献类型：期刊文献

英文题名：Detection of Single Proteins with a General Nanopore Sensor

作者：Li, Qiao[1];Ying, Yi-Lun[1];Liu, Shao-Chuang[1];Lin, Yao[1];Long, Yi-Tao[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]Nanjing Univ, Sch Chem & Chem Engn, Nanjing 210023, Jiangsu, Peoples R China

年份：2019

卷号：4

期号：5

起止页码：1185

外文期刊名：ACS SENSORS

收录：;EI(收录号：20191206673223);WOS:【SCI-EXPANDED(收录号:WOS:000469410100008)】；

基金：This research was supported by the National Natural Science Foundation of China (61871183 and 21834001), Ten Thousand Talent Program for young top-notch talent, Innovation Program of Shanghai Municipal Education Commission (2017-01-07-00-02-E00023), and Shanghai Science and Technology Development Fund (19QA1402300).

语种：英文

外文关键词：solid-state nanopore; single protein detection; cross-linker; dielectric breakdown; functionalization

摘要：Single protein sensing based on solid-state nanopores is promising but challenging, because the fast translocation velocity of a protein is beyond the bandwidth of nanopore instruments. To decelerate the translocation speed, here, we employed a common protein cross-link interaction to achieve a general and robust nanopore sensing platform for single-molecule detection of protein. Benefiting from the EDC/NHS coupling interaction between nanopore and proteins, a 10-fold decrease in speed has been achieved. The clearly distinguishable current signatures further reveal that the anisotropic translocation of a protein, which are horizontal, vertical, and flipping transit inside nanopore confinement. This strategy provides a general platform for rapid detection of proteins as well as exploring fundamental protein dynamics at the single-molecule level.