文献类型：期刊文献

英文题名：Single antibody-antigen interactions monitored via transient ionic current recording using nanopore sensors

作者：Ying, Yi-Lun;Yu, Ru-Jia;Hu, Yong-Xu;Gao, Rui;Long, Yi-Tao[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China; East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：53

期号：61

起止页码：8620

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000406371000029)】；

基金：This research was supported by the National Natural Science Foundation of China (21505043, 21421004 and 21327807) and the Fundamental Research Funds for the Central Universities (222201718001, 222201717003, and 222201714012). Y. L. Ying is supported by the Chinese Post-Doctoral Fund (2016T90340).

语种：英文

摘要：Understanding the single molecular protein-protein interaction has great significance in evaluating the affinity of a specific antibody. Herein, the interaction between single alpha-fetal protein (AFP) and its antibody was monitored via transient ionic current recording by using the antibody functionalized nanopore sensors. More importantly, the kinetic evaluation was performed at the single molecule level to determine the dissociation constant of this interaction. This method enables the monitoring of the kinetic antigen-antibody interaction in their heterogenetic state without any labelling. Our results provided new insights into the evaluation of the antibody's binding affinity and more into the development of immunoassays for diagnostics.