文献类型：期刊文献

英文题名：The potential immunotoxicity of emamectin benzoate on the human THP-1 macrophages

作者：Wei, Ziyi[1];Wang, Weiguo[1];Fu, Wen[1];Zhang, Ping[1];Feng, Hao[1];Xu, Wenping[1];Tao, Liming[1];Li, Zhong[1,2];Zhang, Yang[1];Shao, Xusheng[1,2,3,4]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai Frontier Sci Res Base Optogenet Tech Cel, Shanghai, Peoples R China;[4]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Minist Educ, Shanghai, Peoples R China

年份：2023

卷号：38

期号：3

起止页码：500

外文期刊名：ENVIRONMENTAL TOXICOLOGY

收录：;EI(收录号：20224312997943);WOS:【SCI-EXPANDED(收录号:WOS:000870773000001)】；

基金：National Natural Science Foundation of China, Grant/Award Numbers: 32072441, 21877039; Innovation Program of Shanghai Municipal Education Commission, Grant/Award Number: 2017-01-07-00-02-E00037; National Key Research and Development Program of China, Grant/Award Number: 2018YFD0200100; Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism

语种：英文

外文关键词：emamectin benzoate; immunotoxicity; NF-kappa B pathway; pesticide; THP-1 macrophages

摘要：Emamectin benzoate (EMB) as one of the typical biological pesticides has a wide range of applications in agriculture. However, the immune toxic effects of EMB in human received limited attention. In our study, THP-1 macrophage as an in vitro model was used to evaluate immune functions exposed to EMB. We observed that EMB inhibited phagocytic activity and respiratory burst capacity of macrophages without inducing cellular toxicity, implying the potential immunosuppression. Besides, EMB disturbed the cytokines balance embodied in the increase of TNF-alpha, IL-1 beta, IL-6, CCL27, CXCL8 mRNA expression and the decrease of IL-4, IL-13, 1L-10 mRNA expression. EMB could exhibit pro-inflammatory responses in macrophages and promote the conversion of macrophages to M1 phenotype. Moreover, NF-kappa B pathway involved in regulating immune function from KEGG pathway analysis. EMB exposure could activate the NF-kappa B pathway in THP-1 macrophages by exploring the critical proteins. This research provided insights on immunotoxicity evaluation and clarified EMB-induced immunotoxicity was related to NF-kappa B pathway activation.