文献类型：期刊文献

英文题名：Single nucleotide polymorphism analysis for the production of valuable steroid intermediates in Mycobacterium neoaurum

作者：Liu, Min[1];Zhu, Zhan-Tao[1];Tao, Xin-Yi[1];Wang, Feng-Qing[1];Wei, Dong-Zhi[1]

机构：[1]East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2016

卷号：38

期号：11

起止页码：1881

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20163602768160);WOS:【SCI-EXPANDED(收录号:WOS:000385184600006)】；

基金：This research was financially supported by the Fundamental Research Funds for the Central Universities of China (222201414019), the China Postdoctoral Science Foundation (2014M561427), the National Natural Science Foundation of China (No. 31370080), the National Special Fund for State Key Laboratory of Bioreactor Engineering, the National Basic Research Program of China (973, Program No. 2012CB721003) and the National Major Science and Technology Projects of China (No. 2012ZX09304009).

语种：英文

外文关键词：Deep sequencing; Mycobacterium neoaurum; RNA sequencing; Single nucleotide polymorphism; Sterol transformation; Steroid; Transcriptome

摘要：To investigate single nucleotide polymorphism (SNP) in the transformation process of phytosterol to valuable steroid intermediates in three steroid-producing Mycobacterium neoaurum strains using deep sequencing and bioinformation analysis. The assembled contig sequences from RNA sequencing of strains producing 9 alpha-hydroxy-4-androstene-3,17-dione (9OHAD), 1,4-androstadiene-3,17-dione (ADD), and 22-hydroxy-23, 24-bisnorchola-1,4-dien-3-one (1,4-BNA) were analyzed for the presence of putative SNPs for steroid catabolism. 413, 375, and 491 SNPs were detected in the coding domain sequences and non-coding domain sequences of RNA sequencing reads of M. neoaurum strains producing 9OHAD, ADD, and BNA, respectively. Special attention was focused on SNPs associated with genes showing differential expression at proteome level, including the genes for sterol catabolism, glycerol catabolic process, signal transduction systems, transport system and energy metabolism. The work facilitates the understanding of underlying genetic changes that may be responsible for steroid accumulation in M. neoaurum and is useful for its targeted genetic engineering.