文献类型：期刊文献

中文题名：葡萄糖流加策略对基因工程FR-008/杀念菌素衍生物CS103补料分批发酵过程的影响

英文题名：Influence of glucose feeding strategy on fed-batch cultures of a novel FR-008/candicidin derivatives CS103 by a mutant of Streptomyces sp. FR-008

作者：毛相朝[1];杨亮[2];陈实[3];沈亚领[1];魏东芝[1];邓子新[3]

机构：[1]华东理工大学生物反应器工程国家重点实验室鲁华生物技术研究所,上海200237;[2]华东理工大学生物反应器工程国家重点实验室;[3]上海交通大学微生物代谢教育部重点实验室,上海200030

年份：2009

卷号：39

期号：2

起止页码：1

中文期刊名：工业微生物

外文期刊名：Industrial Microbiology

收录：CSCD:【CSCD2011_2012】；

基金：上海市创新行动计划重大项目(2007DZ19503-4)

语种：中文

中文关键词：葡萄糖;流加策略;补料分批发酵;基因工程FR-008/杀念菌素

外文关键词：glucose; feeding strategy; fed-batch fermentation; novel FR-008/candicidin derivatives

摘要：以组合生物合成技术得到的链霉菌FR-008突变株CS103为研究对象,研究了3.7L发酵罐上维持一定葡萄糖浓度对其次级代谢产物脱羧FR-008/candicidin衍生聚酮抗生素CS103生物合成的影响。当初始葡萄糖浓度20g/L,发酵过程还原糖浓度维持在10g/L时,抗生素CS103最高产量较分批发酵最高产量相比提高30%。研究了3.7L罐上补料分批发酵生产CS103的工艺,主要考察了脉冲补料、间歇流加补料和连续流加补料三种补料分批发酵工艺,并与分批发酵进行了比较。连续流加补料维持糖浓度的效果明显,最高产量达到126.9μg/mL,与分批发酵相比提高了44%左右。
The mutant of Streptomyces sp. FR-008 was conducted through biochemistry method, and its products could be the novel derivatives of polyene macrolides antibiotic FR-008/eanclicidin. The maintained glucose concentration and the corresponding production of the novel antibiotic CS103 were investigated in the 3.7 L fermentor. The optimum substrate of glucose concentration （10 g/L） was obtained for maximal antibiotic CS103 production, increased about 30 96 compared with the batch culture. In fed-batch experiments, pulse-feeding mode, intermittent feeding mode and continuous-feeding mode were compared. The continuous-feeding was observed to be the best strategy than other two feeding modes, in terms of antibiotic yields. The production could reach 126.9 μg/mL, increased about 44% compared with that of the batch culture.