文献类型：期刊文献

英文题名：Production of sesquiterpene patchoulol in mitochondrion-engineered Saccharomyces cerevisiae

作者：Tao, Xin-Yi[1];Lin, Yang-Chen[1];Wang, Feng-Qing[1];Liu, Qing-Hai[1];Ma, Yu-Shu[1];Liu, Min[1];Wei, Dong-Zhi[1]

机构：[1]East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2022

卷号：44

期号：4

起止页码：571

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20221111775424);WOS:【SCI-EXPANDED(收录号:WOS:000765681100001)】；

基金：This study was supported by National Key Research and Development Program of China (2021YFC2100300), the National Natural Science Foundation of China (No. 31500043), the Natural Science Foundation of Shanghai (No. 21ZR1417200), and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Patchoulol; Mitochondria compartmentation; S; cerevisiae; Metabolic engineering; Sesquiterpene

摘要：Patchoulol is a natural sesquiterpene, which is widely used in perfumes and cosmetics. In the work, the mitochondria of S. cerevisiae were engineered for patchoulol production. The patchoulol titer of mitochondria-compartmentalized strain (1.79 mg/L) was 2.71-fold higher than that of control strain (0.66 mg/L) using genome-integrated patchoulol synthase, indicating that mitochondria compartmentation resulted in higher concentration of FPP (farnesyl pyrophosphate) precursor for patchoulol production. Moreover, when fused FPP synthase and patchoulol synthase was overexpressed in the strain with a mitochondria-localized DMAPP (dimethylallyl diphosphate) pathway, the production of patchoulol increased significantly to 19.24 mg/L, indicating more precursors were provided for patchoulol production. Nevertheless, the introduction of excess foreign proteins into mitochondria might cause a certain stress on mitochondria and showed a negative effect on the growth of yeast cells, which could hinder the expression of foreign pathways and reduce the patchoulol production. In conclusion, mitochondria-engineered yeast cells showed important potential for the enhanced biosynthesis of patchoulol, and further engineering could be considered based on the present work.