文献类型：期刊文献

英文题名：Simultaneous saccharification and microbial lipid fermentation of corn stover by oleaginous yeast Trichosporon cutaneum

作者：Liu, Wei[1];Wang, Yumei[1];Yu, Zhanchun[2];Bao, Jie[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Jilin Fuel Alcohol Co, PetroChina Corp, Jilin Econ Dev Zone, Jilin 132101, Peoples R China

年份：2012

卷号：118

起止页码：13

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000307259600003)】；

基金：This research was supported by National Basic Research Program of China (2011CB707406), Natural Science Foundation of China (20976051), the Fundamental Research Funds for the Central Universities of China (WF0913005/1114054), and the Shanghai Leading Academic Discipline Project (B505).

语种：英文

外文关键词：Microbial lipid; Simultaneous saccharification and fermentation (SSF); Corn stover; Trichosporon cutaneum; Cellulase recycling

摘要：Simultaneous saccharification and fermentation (SSF) is the most commonly practiced operation in lignocellulose bioconversion to avoid the sugar product inhibition to cellulose enzymes. In this study, for the first time SSF was tested on microbial lipid fermentation using the diluted acid pretreated and bio-detoxified corn stover. The results show that SSF was effective than the separate hydrolysis and fermentation (SHF) on lipid accumulation of Trichosporon cutaneum CX1 cells in both the small scale (5 L) and the enlarged scale (50 L) bioreactors. The solutions for the oxygen transfer and the lipid extraction in SSF practically worked well. The process parameters were optimized and the lipid yield obtained were 3.03 g/L in the 5 L, and 3.23 g/L in the 50 L, respectively. The result also shows that the cellulose enzyme could be partially recycled in the SSF. The study provided a practical and efficient way for microbial lipid production from lignocellulose material. (C) 2012 Elsevier Ltd. All rights reserved.