文献类型：期刊文献

英文题名：The glutamate biosensor and its application to flow injection analysis system.

作者：Ye, B[1];Li, Q;Li, Y;Yu, J

机构：[1]Research Institute of Biochemical Engineering, East China University of Science and Technology, Shanghai.

年份：1994

卷号：10

期号：2

外文期刊名：Chinese journal of biotechnology

收录：MEDLINE(收录号：7803693);;PubMed

语种：英文

摘要：A micro-enzyme electrode was fabricated by cross-linking L-glutamate oxidase with glutaraldehyde on aminopropyl-platinized platinum wire. A flow injection analysis system with glutamate sensor was used for L-glutamate determination. The peak current is linearly related to the L-glutamate concentration in the range of 0.02-2.0 mM, with good performance, accuracy (CV = 0.4%), fast response (< 60s), and stability (> 20 days). The system was applied to determine the concentration of L-glutamate in a fermentation broth. The recovery rate was in the range of 98.7-107.5%.