文献类型：期刊文献

英文题名：Aminirod propionatiphilus gen. nov., sp. nov., an isolated secondary fermenter in methanogenic hydrocarbon-degrading communities

作者：Liu, Yi-Fan[1,2,4,5];Liu, Zhong-Lin[1,2,4];Ye, Yang-Li[1,2,4];Zhou, Lei[1,2,4];Liu, Jin-Feng[1,2,4];Yang, Shi-Zhong[1,2,4];Gu, Ji-Dong[3];Mu, Bo-Zhong[1,2,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Guangdong Tech Israel Inst Technol, Environm Sci & Engn Res Grp, 241 Daxue Rd, Shantou 515063, Guangdong, Peoples R China;[4]East China Univ Sci & Technol, Engn Res Ctr MEOR, 130 Meilong Rd, Shanghai 200237, Peoples R China;[5]Shanghai Inst Pollut Control & Ecol Secur, Shanghai 200092, Peoples R China

年份：2021

卷号：165

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;EI(收录号：20213710890958);WOS:【SCI-EXPANDED(收录号:WOS:000706182600003)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 41807324, 4201101056 and 41530318), the Natural Science Foundation of Shanghai (Grant No. 21ZR1417400), Fundamental Research Funds for the Central Universities (No. JKJ012016028) and the Research Program of State Key Laboratory of Bioreactor Engineering to Y.F.L. and B.Z.M, the NSFC/RGC Joint Research Fund (No. 41161160560) to J.D.G.

语种：英文

外文关键词：Methanogenic hydrocarbon degradation; Synergistetes; Secondary fermenter; Syntrophic metabolism; Bioreactor; Digestor

摘要：During microbial methanogenic hydrocarbon degradation, secondary fermenters that convert the substrates produced by primary fermenters to acetate, carbon dioxide, hydrogen, and formate which could be further transformed into methane by terminal methanogens have received less attention. In this study, we isolated an anaerobic bacterium, designated strain L-13(T), from a methanogenic crude oil-degrading enrichment culture. The motile, non-sporulating, tenuous rod cells (0.5-0.8 um) stained Gram-positive and grew optimally at 37 degrees C and pH 7.2 with 0.5% NaCl. 16S rRNA gene sequence analysis revealed that strain L-13(T) was distantly related to members of the genus Aminiphilus (89% similarity) in the family Aminiphilaceae of the phylum Synergistetes, and represented a novel genus-level Glade, previously known as EBM-39. Fermentative growth with various amino acids, propionate and some specific sugars was observed, with acetate and hydrogen as the main end products. Syntrophic growth occurred with propionate, butyrate and caprylate in the presence of an H-2-utilizing methanogen, and growth on butyrate and caprylate was only observed under syntrophic conditions. Therefore, this strain may play roles as both secondary fermenter and biomass scavenger in the engineered anaerobic bioreactors/digestors and laboratory enrichments, where primary fermentation products and necromass are readily available. Based on chemotaxonomic, phylogenetic and genomic characteristics, strain L-13(T) (=CGMCC 1.17908) was identified and nominated as Aminirod propionatiphilus gen. nov., sp. nov.