文献类型：期刊文献

英文题名：Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste

作者：Wu, Mengqi[1];Li, Wei[2];Zhang, Yilin[1];Shi, Lei[1];Xu, Zhizhen[1];Xia, Wei[1];Zhang, Wenqing[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Bright Dairy & Food Co Ltd, Dairy Res Inst, State Key Lab Dairy Biotechnol, Shanghai Engn Res Ctr Dairy Biotechnol, Shanghai 200436, Peoples R China

年份：2021

卷号：253

外文期刊名：CARBOHYDRATE POLYMERS

收录：;EI(收录号：20204309387044);WOS:【SCI-EXPANDED(收录号:WOS:000596863400005)】；

基金：This work was supported by the Scientific Fund (19DZ2281400) of Shanghai Engineering Center of Dairy Biotechnology. The authors thank the Shanghai Fangyuan Natural Flavor Co., Ltd. (Shanghai, China) for kindly providing the Rosa Setate x Rosa Rugosa wastes.

语种：英文

外文关键词：Pectic polysaccharides; Physicochemical properties; Hypoglycemic activity; Immunomodulatory activity; Rosa setate x Rosa rugosa wastes

摘要：In this study, two polysaccharide components named WSRP-2a and WSRP-2b, were purified from Rosa setate x Rosa rugosa waste via anion exchange and gel filtration chromatography. Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis confirmed that both of these fractions were mainly composed of galacturonic acid, arabinose, galactose and rhamnose, which were pectin-type polysaccharides with non-triple helix structure. WSRP-2a and WSRP-2b, however, differed in molecular weight of 56.8 kD and 23.9 kD. The followed bioassay presented their impressive hypoglycemic and immunomodulatory activities. WSRP-2a promoted more proliferation, NO release, and the secretion of cytokines in RAW264.7 macrophages, while WSRP-2b presented higher alpha-amylase and alpha-glucosidase inhibitory activities. Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.