文献类型：期刊文献

英文题名：Synthesis of poly-α/β-peptides with tunable sequence via the copolymerization on N-carboxyanhydride and N-thiocarboxyanhydride

作者：Zhou, Min[1];Zou, Jingcheng[2];Liu, Longqiang[2];Xiao, Ximian[2];Deng, Shuai[2];Wu, Yueming[2];Xie, Jiayang[2];Cong, Zihao[2];Ji, Zhemin[2];Liu, Runhui[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Res Ctr Biomed Mat, Frontiers Sci Ctr Mat & Dynam Chem, Minist Educ,Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China

年份：2021

卷号：24

期号：10

外文期刊名：ISCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000713816200001)】；

基金：This research was supported by the National Natural Science Foundation of China (No. 22075078, 21774031, 21861162010, 31800801) , Program of Shanghai Academic/Technology Research Leader (20XD1421400) , Research program of State Key Laboratory of Bioreactor Engineering, the Fundamental Research Funds for the Central Universities (JKD01211520) , and the Frontier Science Research Base of Op-togenetic Techniques for Cell Metabolism grant 2021 Sci & Tech 03-28 (Shanghai Municipal Education Commission) . We thank the Research Center of Analysis and Test of East China University of Science and Technology for the help with the characterization. We also thank the staff members of the MassSpectrometry System at the National Facility for Protein Science in Shanghai (NFPS) , Zhangjiang Lab, China for providing technical support and assistance in data collection and analysis.

语种：英文

摘要：The fascinating functions of proteins and peptides in biological systems have attracted intense interest to explore their mimics using polymers, including polypeptides synthesized from polymerization. The folding, structures and functions of proteins and polypeptides are largely dependent on their sequence. However, sequence-tunable polymerization for polypeptide synthesis is a long-lasting challenge. The application of polypeptides is also greatly hindered by their susceptibility to enzymatic degradation. Although poly-alpha/beta-peptide has proven to be an effective strategy to address the stability issue, the synthesis of poly-alpha/beta-peptide from polymerization is not available yet. Hereby, we demonstrate a living and controlled copolymerization on alpha-NCA and beta-NTA to prepare sequence-tunable poly-alpha/beta-peptides. This polymerization strategy shows a prominent solvent-driven characteristic, providing random-like copolymers of poly-alpha/beta-peptides in THF and block-like copolymers of poly-alpha/beta-peptides in a mixed solvent of CHCl3/H2O (95/5, v/v), and opens new avenues for sequence-tunable polymerization and enables facile synthesis of proteolysis tunable poly-alpha/beta-peptides for diverse applications.