文献类型：期刊文献

中文题名：海洋灰绿曲霉聚酮合酶基因的克隆与序列分析

英文题名：Cloning and Functional Analysis of Fungal Polyketide Synthase Gene from Marine Aspergillus glaucus

作者：李晓霞[1];蔡孟浩[1];胡伟[1];李佳欣[1];周祥山[1];张元兴[1]

机构：[1]华东理工大学生物工程学院,生物反应器工程国家重点实验室,上海200237

年份：2014

卷号：40

期号：5

起止页码：579

中文期刊名：华东理工大学学报(自然科学版)

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家自然科学基金(41306121;31270141);国家863计划(2011AA090702)

语种：中文

中文关键词：灰绿曲霉;聚酮合酶;灰绿霉素A;Agpks1

外文关键词：aspergillus glaucus;polyketide synthase;aspergiolide A;agpksl

摘要：为了研究海洋灰绿曲霉中抗肿瘤聚酮化合物灰绿霉素A的生物合成机制,需获得相关的聚酮合酶基因簇。根据灰绿霉素A合成途径推断,该化合物母核由非还原型PKS(NR-PKS)催化合成,本文使用LC系列简并引物得到灰绿曲霉中NR-PKS基因片段,再通过基因走读技术得到22.8kb的DNA序列,其含有7个完整的开放阅读框。通过BLAST分析,序列中包含1个NRPKS编码基因,全长8451bp,命名为Agpks1。AgPks1与烟曲霉PksP、黑曲霉Alb1有很高的同源性。该工作对研究Agpks1对灰绿霉素A或其他聚酮产物合成的催化机制具有重要意义。
It is critical to identify the polyketide synthase(PKS)gene cluster to clarify the biosynthetic mechanism of antitumor polyketide aspergiolide A,which was deduced to be catalyzed non-reduced PKS(NR-PKS)from marine Aspergillus glaucus.In this study,a NR-PKS nucleotide sequence was first obtained using the LC primers.A full sequence of its gene clusters(22.8 kb)was harvested by genome walking,which contains 70RFs by BLAST analysis.Among them,a NR-PKS gene with full length of 8451 bp was identified by BLAST and designated as Agpksl.The AgPksl showed high homology to Aspergillus furnigatus PksP and Aspergillus niger Albl.This work provides enlightenment for analyzing the functions of Agpksl in biosynthetic mechanism of aspergiolide A or other polyketides produced by Aspergillus glaucus.