文献类型：期刊文献

英文题名：Intramolecular Charge Transfer Inhibition Strategy toward a Desired Solvatochromic Fluorescent Platform: Visualization of Duple Organelles and Detection of Carbon Dioxide

作者：Tian, Minggang[1];Wang, Zhiyuan[1];Zhang, Qilong[1];Wu, Xiaofen[1];Guo, Lifang[2,3];Zheng, Gengxiu[1]

机构：[1]Univ Jinan, Sch Chem & Chem Engn, Jinan 250022, Shandong, Peoples R China;[2]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem, Joint Int Res Lab Precis Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2024

卷号：96

期号：43

起止页码：17290

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20244517304475);WOS:【SCI-EXPANDED(收录号:WOS:001340131400001)】；

基金：For financial support, we thank the National Natural Science Foundation of China (NSFC) (22004036, 22174052, and 21804052) and the Natural Science Foundation of Shandong Province (ZR2021YQ05).

语种：英文

外文关键词：Stearic acid

摘要：Solvatochromic fluorescent probes are crucial molecular tools to investigate and aggregate proteins' fold, visualize fine structures in biomembranes, and label different organelles in dual emission colors. However, solvatochromic fluorogens often displayed a weak emission at high polarity, hindering their bioimaging applications. To resolve this problem, herein, we propose an intramolecular charge transfer (ICT) inhibition strategy. The probe was designed with a single electronic donor and two acceptors in order to split and inhibit the ICT procedure. As a result, the probe displayed an intense emission at both low and high polarities and showed a large emission shift (84 nm) upon polarity change. Using the probe, we successfully imaged lipid droplets and the endoplasmic reticulum in different fluorescence colors. Moreover, the different degrees of lipid accumulation by oleic acid, stearic acid, and cholesterol (oleic acid > stearic acid > cholesterol) have been revealed. The lipid accumulation induced by the three lipids could be rapidly consumed under lipid-less conditions, and the lipids with stearic acid were the most difficult to be consumed. The biological results could facilitate the understanding and treatment of lipid accumulation and obesity. Furthermore, utilizing the polarity increase of diethylamine after the reaction with CO2, the ratiometric detection of CO2 has been achieved for the first time with the probe.