文献类型：期刊文献

英文题名：Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae

作者：Fang, Zhijia[1];Chen, Zhongxiang[1];Wang, Song[2];Shi, Ping[2];Shen, Yuhu[3];Zhang, Youshang[1];Xiao, Junhua[1];Huang, Zhiwei[1]

机构：[1]Donghua Univ, Coll Chem Chem Engn & Biotechnol, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Bioreactor Engn, Shanghai, Peoples R China;[3]Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Qinghai Provinc, Peoples R China

年份：2017

卷号：83

期号：1

外文期刊名：APPLIED AND ENVIRONMENTAL MICROBIOLOGY

收录：;EI(收录号：20170403279166);WOS:【SCI-EXPANDED(收录号:WOS:000393205400006)】；

基金：This work was sponsored by grants from the Natural Science Foundation of Shanghai (grant 15ZR1400200), the Shanghai Scientific and Technological Innovation Project (grant 14520720700), State Education Ministry and Fundamental Research Funds for the Central Universities (grants 2232014A3-03 and 222201313010), and the Applied Basic Research Project of Promotion Plan for Scientific and Technological Innovation in Qinghai Province (grant 2015-ZJ-703).

语种：英文

外文关键词：cadmium; OLE1; delta-9 desaturase; unsaturated fatty acids; cytoplasmic membrane

摘要：The heavy metal cadmium is widely used and released into the environment, posing a severe threat to crops and humans. Saccharomyces cerevisiae is one of the most commonly used organisms in the investigation of environmental metal toxicity. We investigated cadmium stress and the adaptive mechanisms of yeast by screening a genome-wide essential gene overexpression library. A candidate gene, OLE1, encodes a delta-9 desaturase and was associated with high anti-cadmium-stress activity. The results demonstrated that the expression of OLE1 was positively correlated with cadmium stress tolerance and induction was independent of Mga2p and Spt23p (important regulatory factors for OLE1). Moreover, in response to cadmium stress, cellular levels of monounsaturated fatty acids were increased. The addition of exogenous unsaturated fatty acids simulated overexpression of OLE1, leading to cadmium resistance. Such regulation of OLE1 in the synthesis of unsaturated fatty acids may serve as a positive feedback mechanism to help cells counter the lipid peroxidation and cytoplasmic membrane damage caused by cadmium. IMPORTANCE A S. cerevisiae gene encoding a delta-9 desaturase, OLE1, was associated with high anti-cadmium-stress activity. The data suggest that the regulation of OLE1 in the synthesis of unsaturated fatty acids may serve as a positive feedback mechanism to help yeast cells counter the lipid peroxidation and cytoplasmic membrane damage caused by cadmium. The discovery of OLE1 involvement in membrane stability may indicate a novel defense strategy against cadmium stress.