文献类型：期刊文献

中文题名：普通小球藻异养-光自养串联培养的培养基

英文题名：Medium for Culturing Chlorella vulgaris with Sequential Heterotrophic-Autotrophic Model

作者：李兴武[1];李元广[1];沈国敏[1];杨东[2]

机构：[1]生物反应器工程国家重点实验室华东理工大学海洋生化工程研究所,上海200237;[2]上海泽元海洋生物技术有限公司,上海200237

年份：2006

卷号：6

期号：2

起止页码：277

中文期刊名：过程工程学报

外文期刊名：The Chinese Journal of Process Engineering

收录：CSTPCD;;EI(收录号：2006219898246);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家863计划海洋生物技术主题基金资助项目(编号:2004AA626040)

语种：中文

中文关键词：异养-光自养串联培养;普通小球藻;培养基;蛋白质含量;叶绿素含量

外文关键词：sequential heterotrophic-autotrophic cultivation; Chlorella vulgaris; medium; protein content; chlorophyll content

摘要：采用单因素实验和均匀实验获得了适合于普通小球藻异养-光自养串联培养的培养基(HA-SK培养基),其关键是C/N比和保证足够的C,N供应.采用该培养基在摇瓶中异养-光自养串联培养普通小球藻,异养培养结束时细胞密度达13.17g/L,经过36h光自养培养后藻体蛋白质和叶绿素含量分别达49.75%和30.17mg/g.用5L生物反应器和1L平板光生物反应器串联培养,藻细胞密度最高可达15.36g/L,藻体蛋白质和叶绿素含量分别达54.78%和31.23mg/g.表明采用HA-SK培养基进行异养-光自养串联培养可实现普通小球藻的高密度高品质培养.
The medium （HA-SK medium） for culturing Chlorella vulgaris with sequential heterotrophic-autotrophic model was optimized by using the one-at-a-time strategy and uniformity experimental design. When Chlorella vulgaris was cultured with HA-SK medium in 250 mL flask, the cell density reached 13.17 g/L at the end of heterotrophic cultivation, the cellular protein and chlorophyll content were 49.75% and 30.17 mg/g after 36 h autotrophic cultivation, which were increased by 134%, 69.8% and 68.7% respectively comparing with those obtained in an initial medium. As Chlorella vulgaris was cultured with HA-SK medium in sequential 5 L bioreactor and 1 L plat photo-bioreactor system, the cell density reached 15.36 g/L at the end of heterotrophic cultivation, the cellular protein and chlorophyll content were 54.78% and 31.23 mg/g after 35 h autotrophic cultivation. The results show that the cultivation of Chlorella vulgaris with high cell density and high quality can be realized successfully by sequential heterotrophic-autotrophic model with HA-SK medium.