文献类型：期刊文献

英文题名：Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers

作者：Lu, Ziyi[1];Wu, Yueming[1];Cong, Zihao[1];Qian, Yuxin[1];Wu, Xue[1];Shao, Ning[1];Qiao, Zhongqian[1];Zhang, Haodong[1];She, Yunrui[1];Chen, Kang[1];Xiang, Hengxue[2];Sun, Bin[2];Yu, Qian[3];Yuan, Yuan[5];Lin, Haodong[4];Zhu, Meifang[2];Liu, Runhui[1,5]

机构：[1]East China Univ Sci & Technol, Coll Chem Chem Engn & Mat Sci, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Donghua Univ, Coll Mat Sci & Engn, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 201620, Peoples R China;[3]Soochow Univ, Jiangsu Key Lab Adv Funct Polymers Design & Appli, Suzhou 215123, Peoples R China;[4]Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Orthoped Surg, Sch Med, Shanghai 200080, Peoples R China;[5]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Res Ctr Biomed Mat,Minist Educ, Sch Mat Sci & Engn,Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China

年份：2021

卷号：6

期号：12

起止页码：4531

外文期刊名：BIOACTIVE MATERIALS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000709332400021)】；

基金：This research was supported by the National Natural Science Foundation of China (No. 22075078, 21774031), the National Key Research and Development Program of China (2016YFC1100401), Program of Shanghai Academic/Technology Research Leader (20XD1421400), State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, the Natural Science Foundation of Jiangsu Province (BK20180093), the Natural Science Foundation of Shanghai (18ZR1410300), Research program of State Key Laboratory of Bioreactor Engineering, the Fundamental Research Funds for the Central Universities (22221818014), The authors also thank Research Center of Analysis and Test of East China University of Science and Technology for the help on the characterization.

语种：英文

外文关键词：Peptide polymer; Host defense peptide; Antimicrobial surface; MRSA; Subcutaneous infection

摘要：It is an urgent need to tackle drug-resistance microbial infections that are associated with implantable biomedical devices. Host defense peptide-mimicking polymers have been actively explored in recent years to fight against drug-resistant microbes. Our recent report on lithium hexamethyldisilazide-initiated superfast polymerization on amino acid N-carboxyanhydrides enables the quick synthesis of host defense peptide-mimicking peptide polymers. Here we reported a facile and cost-effective thermoplastic polyurethane (TPU) surface modification of peptide polymer (DLL: BLG = 90 : 10) using plasma surface activation and substitution reaction between thiol and bromide groups. The peptide polymer-modified TPU surfaces exhibited board-spectrum antibacterial property as well as effective contact-killing ability in vitro. Furthermore, the peptide polymer-modified TPU surfaces showed excellent biocompatibility, displaying no hemolysis and cytotoxicity. In vivo study using methicillin-resistant Staphylococcus aureus (MRSA) for subcutaneous implantation infectious model showed that peptide polymer-modified TPU surfaces revealed obvious suppression of infection and great histocompatibility, compared to bare TPU surfaces. We further explored the antimicrobial mechanism of the peptide polymer-modified TPU surfaces, which revealed a surface contact-killing mechanism by disrupting the bacterial membrane. These results demonstrated great potential of the peptide-modified TPU surfaces for practical application to combat bacterial infections that are associated with implantable materials and devices.