文献类型：期刊文献

英文题名：A de novo zwitterionic strategy of ultra-stable chemiluminescent probes: highly selective sensing of singlet oxygen in FDA-approved phototherapy

作者：Lu, Yao[1];Zhang, Yutao[1];Wu, Xia[2];Pu, Ruihua[3];Yan, Chenxu[1];Liu, Weimin[3];Liu, Xiaogang[2];Guo, Zhiqian[1];Zhu, Wei-Hong[1]

机构：[1]East China Univ Sci & Technol, Inst Fine Chem, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem,Sch Che, Shanghai 200237, Peoples R China;[2]Singapore Univ Technol & Design, Fluorescence Res Grp, 8 Somapah Rd, Singapore 487372, Singapore;[3]ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China

年份：2024

卷号：15

期号：31

起止页码：12431

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20242816693143);WOS:【SCI-EXPANDED(收录号:WOS:001268237900001)】；

基金：This work was supported by NSFC/China (32121005, 22225805, 22308101, and 32394001), the National Key Research and Development Program (2021YFA0910000), the Shanghai Science and Technology Innovation Action Plan (No. 23J21901600), the Innovation Program of Shanghai Municipal Education Commission, the Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission, grant 2021 Sci & Tech 03-28), the China Postdoctoral Science Foundation (2021M701199), the Shanghai Municipal Science and Technology Major Project (Grant 2018SHZDZX03), and the Natural Science Foundation of Shanghai (23ZR1416600).

语种：英文

外文关键词：Chemiluminescence - Excited states - Infrared devices - Ketones - Mammals - Medical applications - Oxygen - Photodynamic therapy - Signal to noise ratio

摘要：Singlet oxygen (O-1(2)), as a fundamental hallmark in photodynamic therapy (PDT), enables ground-breaking clinical treatment in ablating tumors and killing germs. However, accurate in vivo monitoring of O-1(2) remains a significant challenge in probe design, with primary difficulties arising from inherent photo-induced side reactions with poor selectivity. Herein, we report a generalizable zwitterionic strategy for ultra-stable near-infrared (NIR) chemiluminescent probes that ensure a highly specific [2 + 2] cycloaddition between fragile electron-rich enolether units and O-1(2) in both cellular and dynamic in vivo domains. Innovatively, zwitterionic chemiluminescence (CL) probes undergo a conversion into an inert ketone excited state with an extremely short lifetime through conical intersection (CI), thereby affording sufficient photostability and suppressing undesired photoreactions. Remarkably, compared with the well-known commercial O-1(2) probe SOSG, the zwitterionic probe QMI exhibited an ultra-high signal-to-noise ratio (SNR, over 40-fold). Of particular significance is that the zwitterionic CL probes demonstrate excellent selectivity, high sensitivity, and outstanding photostability, thereby making a breakthrough in real-time tracking of the FDA-approved 5-ALA-mediated in vivo PDT process in living mice. This innovative zwitterionic strategy paves a new pathway for high-performance NIR chemiluminescent probes and high-fidelity feedback on O-1(2) for future biological and medical applications.