文献类型：期刊文献

英文题名：Improving enzymatic production of ginsenoside Rh2 from Rg 3 by using nonionic surfactant

作者：Su, Jin-Huan[1]; Xu, Jian-He[1]; Wang, Zhi-Long[2]

机构：[1] Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; [2] School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China

年份：2010

卷号：160

期号：4

起止页码：1116

外文期刊名：Applied Biochemistry and Biotechnology

收录：EI(收录号：20101112767969)

语种：英文

外文关键词：Biocompatibility - Fungi - Enzymatic hydrolysis

摘要：In this study, several nonionic surfactants were tried to improve the enzymatic hydrolysis of ginsenoside Rg3 into Rh2 which was catalyzed at 50 °C and pH 5.0 by a crude glucosidase extracted from Fusarium sp. ECU2042. Among the biocompatible nonionic surfactants, polyethylene glycol 350 monomethyl ether was shown to be the best. After optimizing some influencing factors on the reaction, the conversion of Rg3 (5 g/l) with 10 g/l crude enzyme reached almost 100% in the presence of the nonionic surfactant (7.5%, w/v), which was 25% higher than that in buffer without any surfactant. Furthermore, the enzyme stability was affected faintly by the surfactant. ? 2009 Humana Press.