文献类型：期刊文献

英文题名：Formaldehyde reinforces pro-inflammatory responses of macrophages through induction of glycolysis

作者：Ma, Huijuan[1,2];Lin, Jinxuan[1,2];Li, Linyi[1,2];Ding, Zhaoqian[1,2];Huang, Ping[1,2];Song, Xiaodong[3];Lou, Kaiyan[1,2];Wang, Wei[1,2,4,5];Xu, Huan[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Dept Pharmaceut Sci, Shanghai 200237, Peoples R China;[3]Fudan Univ, Hua Shan Hosp North, Dept Lab Med, Shanghai 201907, Peoples R China;[4]Univ Arizona, Dept Pharmacol & Toxicol, Tucson, AZ 85721 USA;[5]Univ Arizona, BIO5 Inst, Tucson, AZ 85721 USA

年份：2021

卷号：282

外文期刊名：CHEMOSPHERE

收录：;EI(收录号：20212410508377);WOS:【SCI-EXPANDED(收录号:WOS:000707307400018)】；

基金：This work was funded by National Natural Science Foundation of China [grant No. 21906057 to H. X., grant No. 21738002 to W. W., grant No.21577037 to K. L.]. This work was also funded by Shanghai Science and Technology Committee through the Shanghai Sailing Program [grant No. 19YF1412500 to H. X.] and the Shanghai Natural Science Fund [grant No. 20ZR1414700 to K. L.].

语种：英文

外文关键词：Formaldehyde; Macrophage; Glycolysis; Pro-inflammatory responses; Lipopolysaccharide stimulation; HIF-1 alpha

摘要：Formaldehyde (FA) is widely used in chemical industry, which is also known as a common indoor air pollutant. Exposure of FA has been associated with multiple detrimental health effects. Our previous study showed that FA could inhibit the development of T lymphocytes in mice, leading to impaired immune functions. Macrophages are important innate immune cells which trigger inflammatory responses in tissues. In the present study, FA exposure at 2.0 mg/m(3) was found to enhance the pro-inflammatory responses of macrophages in male BALB/c mice, which was confirmed by elevated pro-inflammatory cytokine release and NO secretion in macrophages isolated from the FA-exposed mice and in vitro macrophage models upon lipopolysaccharide stimulation. Glycolysis is the key metabolic process for the classical activation of macrophages, which was found to be elevated in the in vitro macrophage models treated with FA at 50 and 100 mu M concentrations for 18 h. HIF-1 alpha and the associated proteins in its signaling cascade, which are known to mediate glycolytic metabolism and inflammatory responses, were found to be upregulated by 50 and 100 mu M FA in THP-1 derived and RAW264.7 macrophage models, and the enhanced pro-inflammatory responses induced by 100 mu M FA were reversed by inhibitory compounds interfering with glucose metabolism or suppressing HIF-1 alpha activity. Collectively, the results in this study revealed that FA could enhance the pro-inflammatory responses of macrophages through the induction of glycolysis, which outlined the FA-triggered metabolic and functional alterations in immune cells.