文献类型：期刊文献

中文题名：异羟肟酸比色法测定D-核糖发酵液中葡萄糖酸的含量

英文题名：Determination of Gluconate Concentration in Broth of D-ribose Fermentation Using the Hydroxamate Method

作者：张宗玉[1];李志敏[1];叶勤[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室

年份：2005

卷号：31

期号：8

起止页码：81

中文期刊名：食品与发酵工业

外文期刊名：Food and Fermentation Industries

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：葡萄糖酸;异羟肟酸比色法;葡萄糖;比色法测定;异羟肟酸;D-核糖;糖酸含量;发酵液;葡萄糖浓度;测定影响;检测结果

外文关键词：gluconate, hydroxamate colorimetric method, glucose

摘要：对异羟肟酸比色法测定葡萄糖酸含量的方法进行了改进。通过考察不同葡萄糖浓度对异羟肟酸比色法测定葡萄糖酸的影响,得出葡萄糖浓度对葡萄糖酸测定影响的干扰方程,据此计算得到的葡萄糖酸量可以排除葡萄糖的影响,使得检测结果更为准确。方法适用于D-核糖发酵液中葡萄糖酸的测定。
Glucose interferes with gluconate measurement using the hydroxamate colorimetric method. This study focused on the effect of glucose on the measurement of gluconate. Through measuring a series of solutions containing different amount of glucose and gluconate, a formula for calculating gluconate concentration was obtained, with which the interference caused by glucose could be eliminated. This method was applied to determination of gluconate concentration profile during D- ribose fermentation.