文献类型：期刊文献

英文题名：Effective cancer immunotherapy by Ganoderma lucidum polysaccharide-gold nanocomposites through dendritic cell activation and memory T cell response

作者：Zhang, Shulei[1];Pang, Guibin[1];Chen, Chao[3];Qin, Jianzhong[4];Yu, Huan[2];Liu, Yongming[2];Zhang, Xihui[2];Song, Zhentao[6];Zhao, Jian[6];Wang, Fujun[1,5,7];Wang, Yangyun[2];Zhang, Leshuai W.[2,5]

机构：[1]Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, 1200 Cailun Rd, Shanghai 201203, Peoples R China;[2]Soochow Univ, Sch Radiol & Interdisciplinary Sci RAD X, State Key Lab Radiat Med & Protect, Sch Radiat Med & Protect,Collaborat Innovat Ctr R, Suzhou 215123, Peoples R China;[3]Changshu 2 Peoples Hosp, Dept Gen Surg, Changshu 215500, Jiangsu, Peoples R China;[4]Soochow Univ, Dept Orthoped, Affiliated Hosp 2, Suzhou 215007, Peoples R China;[5]Zhejiang Reachall Pharmaceut Co Ltd, Dongyang 322100, Zhejiang, Peoples R China;[6]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[7]Shanghai R&D Ctr Standardizat Chinese Med, Shanghai 201210, Peoples R China

年份：2019

卷号：205

起止页码：192

外文期刊名：CARBOHYDRATE POLYMERS

收录：;EI(收录号：20184305980311);WOS:【SCI-EXPANDED(收录号:WOS:000450093200022)】；

基金：This work was supported by National Natural Science Foundation of China (#81373950, #51503139, #31771104), Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection for financial support.

语种：英文

外文关键词：Polysaccharides; Dendritic cell; Nanoparticle; Immunoregulation; Cancer therapy

摘要：Polysaccharides purified from natural herbs possess immunoregulatory functions, while the efficacy of natural polysaccharides on cancer treatment remains unreliable, likely due to their low prescribed doses and fast clearances in clinical settings. In this study, gold nanocomposites containing Ganoderma lucidum polysaccharide (GLP-Au) efficiently induced dendritic cell (DC) activation, evident by the increase of CD80/CD86/CD40/MHCII, decrease of phagocytic ability and acid phosphatase activity, and increased cytokine transcription. GLPAu significantly promoted the proliferation of CD4 + and CDS+ T cells in splenocytes. DC/T cell co-culture study proved that GLP-Au activation on DC directly resulted in T cell proliferation. GLP-Au exhibited strong inhibitory effects on 4T1 tumor growth and pulmonary metastasis when combined with doxorubicin. GLP-Au recovered body weight loss by doxorubicin and increased the percentage of CD4 + /CD44 + memory T cells. This work suggests that polysaccharides from natural herbs can be incorporated into nanocomposites with immunoregulatory characteristics for enhanced efficacy on tumor therapy.