文献类型：期刊文献

英文题名：Three sulphated polysaccharides isolated from the mucilage of mud snail, Bullacta exarata philippi: Characterization and antitumour activity

作者：Zhang, Daihui[2];Wang, Chong[2];Wu, Hongxi[3];Xie, Jingli[1,2];Du, Lei[2];Xia, Zhaoming[2];Cai, Jingbo[3];Huang, Zhenhua[3];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Dept Food Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China;[3]Zhejiang Mariculture Res Inst, Zhejiang Key Lab Coastal Bioresource, Wenzhou 325005, Peoples R China

年份：2013

卷号：138

期号：1

起止页码：306

外文期刊名：FOOD CHEMISTRY

收录：;EI(收录号：20130115867840);WOS:【SCI-EXPANDED(收录号:WOS:000314193800046)】；

基金：This work was supported by "The Fundamental Research Funds for the Central Universities" and "The Open Project Funding of Zhejiang Key Laboratory of Coastal Bio-resource", P. R. China.

语种：英文

外文关键词：Bullacta exarata; Mucilage; Sulphated polysaccharides; Structure; Antitumour activity

摘要：Three sulphated polysaccharides, coded as BEMPA, BEMPB1, BEMPB2, were extracted from the mucilage of mud snail of Bullacta exarata and purified by DEAE-cellulose ion-exchange and size-exclusion chromatography. Structural analysis of purified polysaccharides by chemical and biochemical methods revealed BEMPA was a high (1 -> 3,4)-linked mannose-containing polysaccharide with molecular weight of 22,977 Da. BEMPB1, with molecular weight of 64,117 Da, was a high (1 -> 3)-linked arabinose-containing polysaccharide. BEMPB2 was mainly composed of (1 -> 3,4)-linked mannose with molecular weight of 47,507 Da. The comparison between sulphated polysaccharides and their desulphated products showed that sulphate substitutions of BEMPB1 were deduced to be at the C-3 of (1 -> 4)-linked mannose, while sulphate substitutions of BEMPA and BEMPB2 were at C-4 of (1 -> 3)-linked mannose. Furthermore, BEMPA exhibited highest inhibitory effects on growth of B-16 melanoma cells, and IC50 were 31.1 mu g/mL. (C) 2012 Elsevier Ltd. All rights reserved.