文献类型：期刊文献

英文题名：Avermectin induces developmental toxicity and immunotoxicity in zebrafish larvae

作者：Wang, Wei-Guo[1];Li, Shou-Lin[1];Yan, Da-Wei[2];Sha, Yun-Fei[3];Xu, Wen-Ping[1];Cheng, Jia-Gao[1];Li, Zhong[1];Tao, Li-Ming[1];Zhang, Yang[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Shanghai New Tobacco Prod Res Inst Co Ltd, Shanghai 201315, Peoples R China;[3]Shanghai Tobacco Grp Co Ltd, New Tobacco Prod Engn Ctr, Shanghai 201315, Peoples R China

年份：2026

卷号：168

起止页码：729

外文期刊名：JOURNAL OF ENVIRONMENTAL SCIENCES

收录：;EI(收录号：20263021175021);Scopus(收录号：2-s2.0-105045567286);WOS:【SCI-EXPANDED(收录号:WOS:001837334200001)】；

基金：This work was supported by the State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China (No. SKL-GPL-KF-202404).

语种：英文

外文关键词：Avermectin; Immunotoxicity; Developmental toxicity; Zebrafish larvae

摘要：Avermectin has been widely used in agriculture and animal husbandry for decades, and its associated environ-mental problems have raised increasing concerns regarding its safety. This study investigated the developmental and immunotoxicity of avermectin in zebrafish larvae and its underlying mechanisms. The results showed that exposure to 0.15-0.60 mu g/mL avermectin for 72 h induced developmental malformations in zebrafish larvae, including decreased heart rate, spinal curvature, and edema. It also significantly reduced the numbers of immune cells such as neutrophils, T cells, and macrophages. At the molecular level, avermectin upregulated the levels of nitric oxide (NO) and inflammatory cytokines (TNF-alpha, IL-1 beta, IL-6), and activated the TLR4/MyD88/NF-kappa B in-flammatory signaling pathway. Transcriptomic analysis identified 1085 differentially expressed genes, and KEGG enrichment analysis suggested that signaling pathways such as the complement and coagulation cascades, IL-17 signaling pathway, and Toll-like receptor signaling pathway may collectively mediate the immunotoxicity. In conclusion, avermectin causes multiple toxic injuries in zebrafish larvae by triggering excessive inflammatory responses and immunosuppression.