文献类型：期刊文献

中文题名：桑叶多糖SJB的结构分析和蛋白酪氨酸磷酸酯酶PTP1B抑制活性

英文题名：Structural Analysis of Polysaccharide SJB from Mulberry Leaves and Its Inhibitory Activities of Protein Tyrosine Phosphatase-1B

作者：夏玮[1];张文清[1];罗国安[2]

机构：[1]华东理工大学化学与分子工程学院,上海200237;[2]清华大学化学系,北京100084

年份：2008

卷号：29

期号：11

起止页码：2205

中文期刊名：高等学校化学学报

外文期刊名：Chemical Journal of Chinese Universities

收录：CSTPCD;;EI(收录号：20090111826603);Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000261462700018)】；北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：上海市重点学科建设项目资金(批准号:B507)资助

语种：中文

中文关键词：桑叶;多糖;结构鉴定;蛋白酪氨酸磷酸酯酶PTP1B抑制活性

外文关键词：Mulberry leaves; Pectic polysaccharide; Structure analysis; Inhibitory activities of protein tyrosine phosphatase-1B

摘要：通过DEAE-纤维素和凝胶过滤柱色谱对桑叶碱提粗多糖进行分级分离,获得均一多糖SJB,进行结构鉴定.采用蛋白酪氨酸磷酸酯酶PTP1B体外模型对SJB进行降血糖活性测定.结果表明:SJB的相对分子质量为5.4×104,由鼠李糖、阿拉伯糖、葡萄糖、半乳糖、半乳糖醛酸组成的酸性杂多糖;主链由1,2-、1,2,4-连接的鼠李糖和1,4-、1,3,4-连接的半乳糖醛酸组成;侧链包括末端、1,5-、1,3,5-连接的阿拉伯糖;末端、1,4-连接的葡萄糖以及末端、1,3-、1,4-、1,6-连接的半乳糖,主要通过鼠李糖的O4位和半乳糖醛酸的O3位与主链相连.该多糖为首次从桑叶中获得的酸性杂多糖.20μg/mL SJB对PTP1B的抑制率为31.7%.
A pectic polysaccharide SJB was obtained from the alkali extract of mulberry leaves by DEAE-cellulose and gel filtration chromatography. The structures of SJB were identified on the basis of chemical methods （sugar component analysis, methylation analysis, partial acid hydrolysis） and spectral method（ IR, ESI-MS, NMR）. The results show that SJB with a molecular mass of 5.4 × 10^4 consisted of rhammose, arabinose, glucose, galactose and galacturonic acid units in the molar ratio of 1.00： 1.26： 0.66： 2.04： 0.95. The main chain of SJB includes 1,2-1inked, 1,2,4-1inked Rhap and 1,4-1inked, 1,3,4-1inked GalAp. The side chains are mainly attached to the 04 of Rhap residues and 03 of GalAp residues, including terminal, 1,5-1inked, 1,3,5-linked Arafand terminal, 1,4-1inked Glcp, and terminal, 1,4-, 1,3-, 1,6-linked Galp. SJB is a new acidic polysaccharide for the first time isolated from Muberry leaves. The study of the effect of lowering blood glucose of SJB shows the inhibitory rate of SJB to PTP1B is 31.7% at the concentration of 20 μg/mL.