文献类型：期刊文献

中文题名：三七悬浮细胞生物合成绿原酸类物质及其生物活性

英文题名：Biosynthesis and Bioactivity of Chlorogenic Acids in Panax notoginseng Suspension Cells

作者：周倍[1];刘楠[1];刘泽波[1];庄英萍[1];王泽建[1];郭美锦[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2023

卷号：49

期号：4

起止页码：515

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：Scopus;北大核心:【北大核心2020】；CSCD:【CSCD_E2023_2024】；

基金：国家重点研发计划(2020YFA0906800)。

语种：中文

中文关键词：三七愈伤组织细胞;悬浮培养;绿原酸生物合成;工艺优化;生物活性

外文关键词：Panax notoginseng calli;suspension culture;chlorogenic acid biosynthesis;process optimization;bioactivity

摘要：利用三七(Panax notoginseng)悬浮细胞生物合成绿原酸类物质(CGAs),并对其生物活性进行了研究。对三七细胞中CGAs进行定性分析,优化了细胞培养条件以及6种诱导模式,测定了三七细胞CGAs的抗氧化及α-葡萄糖苷酶活性抑制能力。结果表明三七愈伤组织内含有4种CGAs;最优工艺条件为:B5+40 g/L蔗糖,pH 7.5,15%接种量;经30μmol/L茉莉酸甲酯诱导3 d后,CGAs产量(684.07 mg/L)比原工艺(475.18 mg/L)提高了0.44倍;三七细胞CGAs具有良好的抗氧化及α-葡萄糖苷酶活性抑制能力。
In this paper,Panax notoginseng(P.notoginseng)suspension cells were used for the production of chlorogenic acids(CGAs),of which bioactivities were evaluated.Firstly,CGAs in P.notoginseng suspension cells were identified by liquid chromatography-tandem mass spectrometry.Secondly,the suspension culture system and elicitation mode were optimized.Ultimately,the antioxidant andα-glucosidase inhibitory activity of CGAs from P.notoginseng cells were determined.Four CGAs were inferred in P.notoginseng cells.The optimal condition was obtained(B5+40 g/L sucrose,pH 7.5 and 15%inoculation size).By co-culture with 30μmol/L methyl jasmonate for 3 d,the CGAs yield could reach up to 684.07 mg/L,which was 1.44 times that of the control.CGAs from P.notoginseng cells showed an excellent antioxidant capacity in radical-scavenging test and,moreover,certain inhibition onα-glucosidase activity.Our results indicate that the production of CGAs by P.notoginseng cells has potential applications in healthy food and daily cosmetics.