文献类型：期刊文献

英文题名：Programming Rotary Motions with a Hexagonal DNA Nanomachine

作者：Yang, Yangyang[1];Zhang, Shiwei[1];Yao, Shengtao[1];Pan, Rizhao[1];Hidaka, Kumi[3];Emura, Tomoko[3];Fan, Chunhai[4,5];Sugiyama, Hiroshi[3];Xu, Yufang[1];Endo, Masayuki[3];Qian, Xuhong[1,2]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Kyoto Univ, Dept Chem, Grad Sch Sci, Sakyo Ku, Kyoto 6068501, Japan;[4]Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Renji Hosp, Sch Med, Shanghai 200240, Peoples R China;[5]Shanghai Jiao Tong Univ, Inst Mol Med, Renji Hosp, Sch Med, Shanghai 200240, Peoples R China

年份：2019

卷号：25

期号：20

起止页码：5158

外文期刊名：CHEMISTRY-A EUROPEAN JOURNAL

收录：;EI(收录号：20191206666187);WOS:【SCI-EXPANDED(收录号:WOS:000468855200008)】；

基金：This work was supported by the Shanghai Sailing Program, National Natural Science Foundation of China (Grant No. 31600802), Key Project of the Shanghai Science and Technology Committee (18DZ1112703) and the Fundamental Research Funds for the Central Universities. We thank Prof. B. Li, Prof. L. Wang, Ms W. Liu (Shanghai Institute of Applied Physics, Chinese Academy of Sciences) for the support of the AFM imaging.

语种：英文

外文关键词：biocatalytic cascade reactions; DNA origami; nanodevices; rotary nanomachines

摘要：Biological macromolecular machines perform impressive mechanical movements. F-adenosine triphosphate (ATP) synthase uses a proton gradient to generate ATP through mechanical rotations. Here, a programmed hexagonal DNA nanomachine, in which a three-armed DNA nanostructure (TAN) can perform stepwise rotations in the confined nanospace powered by DNA fuels, is demonstrated. The movement of TAN can precisely go through a 60 degrees rotation, which is confirmed by atomic force microscopy, and each stepwise directional rotating is monitored by fluorescent measurements. Moreover, the rotary nanomachine is used to spatially organize cascade enzymes: glucose oxidase (GOx) and horseradish peroxidase (HRP) in four different arrangements. The multistep regulations of the biocatalytic activities are achieved by employing TAN rotations. This work presents a new prototype of rotary nanodevice with both angular and directional control, and provides a nanoscale mechanical engineering platform for the reactive molecular components, demonstrating that DNA-based framework may have significant roles in futuristic nanofactory construction.