文献类型：期刊文献

英文题名：Risk assessment of antibiotic resistance genes in the drinking water system

作者：Hu, Yaru[1,2];Jiang, Lei[3];Sun, Xiaoyan[4];Wu, Jianqiang[5];Ma, Lei[4];Zhou, Yanbo[1,2];Lin, Kuangfei[1];Luo, Yi[6];Cui, Changzheng[1,2]

机构：[1]East China Univ Sci & Technol, State Environm Protect Key Lab Environm Risk Asse, Sch Resources & Environm Engn, Shanghai 200237, Peoples R China;[2]Shanghai Inst Pollut Control & Ecol Secur, Shanghai 200092, Peoples R China;[3]Natl Engn Res Ctr Urban Water Resources, Shanghai 200082, Peoples R China;[4]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[5]Shanghai Acad Environm Sci, Shanghai 200233, Peoples R China;[6]Nankai Univ, Coll Environm Sci & Engn, Tianjin Key Lab Environm Remediat & Pollut Contro, Minist Educ,Key Lab Pollut Proc & Environm Criter, Tianjin 300071, Peoples R China

年份：2021

卷号：800

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：;EI(收录号：20213410810011);WOS:【SCI-EXPANDED(收录号:WOS:000701672200002)】；

基金：This research was supported by the Science and Technology Commission of Shanghai Municipality (No. 20dz1200800) , the National Water Pollution Control and Management Technology Major Projects (No. 2017ZX07402003) , the Shanghai Sailing Program (No. 21YF1409700) , and the National Natural Science Funds Fund (No. 51679141) .

语种：英文

外文关键词：Antibiotic resistance genes; Chlorine-resistant; Drinking water system; Resistance risk ranking in drinking water system ((RDW)-D-3); Risk assessment; Trace level antibiotics

摘要：Antibiotic resistance genes (ARGs) are extensively detected in various environmental media, whose risk assessment in the drinking water systems has not been comprehensive. This study established a new risk assessment of ARGs in the drinking water systems, considering the chlorine-resistance ability, transferability, and ARGs harboring potential of pathogens. The risk of ARGs in a typical drinking water reservoir was also evaluated based on the detection of ARGs and antibiotic-resistant bacteria (ARB). Fourteen ARGs were detected with a relative concentration range of 10(-4)-10(-3) (ARGs/16S rRNA gene). Five isolated ARB were identified as human opportunistic pathogens, one of which (Pseudomonas aeruginosa HLS-6, CCTCC AB 2017269) is resistant to hundreds of milligrams per liter levels of antibiotics and low-level chlorine. This result indicated that ARB tolerant to high-levels of antibiotics could be isolated from environments containing trace levels of antibiotics. Moreover, complete genome sequencing confirmed the inclusion of ARGs (sul1, aadA2) on the class I integron in HLS-6, indicating that the risk of ARGs in this drinking water reservoir could be classified as resistance risk ranking in drinking water system 1 ((RDW)-D-3 1). The risk assessment of ARGs in this study provides a clear understanding of ARG risk in drinking water systems. The results reveal that the ARGs and ARB contamination of drinking water reservoirs pose significant challenges for drinking water treatment efficiency and affect drinking water safety. (C) 2021 Elsevier B.V. All rights reserved.