文献类型：期刊文献

英文题名：Quality evaluation of Platycladus orientalis (L.) Franco through simultaneous determination of four bioactive flavonoids by high-performance liquid chromatography

作者：Lu, Yan-hua; Liu, Zhi-yong; Wang, Zheng-tao; Wei, Dong-zhi

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Univ TCM, Inst Chinese Mat Med, Shanghai 201203, Peoples R China

年份：2006

卷号：41

期号：4

起止页码：1186

外文期刊名：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000238491100015)】；

语种：英文

外文关键词：Platycladus orientalis (L.) Franco (Cupressaceae); rutin; quercitrin; quercetin; amentoflavone; flavonoid; HPLC/DAD

摘要：Platycladus orientalis (L.) Franco (Cupressaceae), a traditional Chinese herb and food additive, has been used for treatments of gout, rheumatism, diarrhoea and chronic tracheitis. To evaluate the quality of R orientalis (L.) Franco, a sensitive, simple and accurate reversed-phase high-performance liquid chromatographic (RP-HPLC) separation method with a photodiode array detector (DAD) was developed for the determination of four main bioactive flavonoids, rutin, quercitrin, quercetin and amentoflavone. Separation of the four compounds was achieved by the HPLC assay (Agilent Eclipse XDB-C 18 column with mobile phase, methanol-acetonitrile-18 mM sodium acetate buffer (pH 3.5) and recorded at UV 356 nm). This method showed good linear relation in the range of 0.8-80 mu g/ml for rutin, 1.84-184 mu g/ml for quercitrin, 0.72-72 mu g/ml for quercetin and 0.72-72 mu g/ml for amentoflavone. The correlation coefficients of the calibration curve for the analysis were all higher than 0.999. In addition, the contents of those four flavonoids in P. orientalis (L.) Franco growing in 12 different locations in China were compared to establish the effectiveness of the method. (c) 2006 Elsevier B.V. All rights reserved.