文献类型：期刊文献

英文题名：Simple and efficient preparation of high-purity trehalulose from the waste syrup of isomaltulose production using solid-phase extraction followed by hydrophilic interaction chromatography

作者：Fu, Dongmei[1];Zhang, Xue[1];Zhang, Hao[1];Fu, Qing[2];Jin, Yu[2];Yan, Jingyu[3];Li, Xianzhen[1]

机构：[1]Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Minist Educ, Shanghai, Peoples R China;[3]Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China

年份：2021

卷号：44

期号：12

起止页码：2334

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20211810301158);WOS:【SCI-EXPANDED(收录号:WOS:000646250300001)】；

基金：Natural Science Foundation of Liaoning Province of China, Grant/Award Number: 20180550134; Opening Project ofKey Laboratory of Separation Science for Analytical Chemistry; ChineseAcademy of Sciences, Grant/Award Number: KL1810; Natural Science Foundation of Liaoning Province, Grant/Award Number: 20180550134

语种：英文

外文关键词：hydrophilic interaction chromatography; sucrose isomerase; sugar isomers; tautomer transition; trehalulose

摘要：A simple and efficient method was developed for the preparation of high-purity trehalulose from the waste syrup of isomaltulose production. The waste syrup was pre-treated with C18 solid-phase extraction, where 98% decolorization and 97% reducing sugar recovery were obtained, followed by hydrophilic interaction liquid chromatography separation on a cysteine-bonded zwitterionic column. Under optimized conditions, trehalulose was separated from isomaltulose isomer and prepared on a semi-preparative scale with >99% purity. The structure of the prepared trehalulose was subsequently confirmed by nuclear magnetic resonance, and three tautomers of trehalulose (alpha-D-glucosylpyranosyl-1,1-beta-D-fructopyranose, alpha-D-glucosylpyranosyl-1,1-beta-D-fructofuranose, and alpha-D-glucosylpyranosyl-1,1-alpha-D-fructofuranose) were detected and completely characterized by C-13 NMR spectroscopy for the first time in this study. The tautomerization of alpha-D and beta-D type transition was observed by hydrophilic interaction liquid chromatography on an AdvanceBio Glycan Mapping column, with smaller particle size (2.7 mu m). Furthermore, the prepared trehalulose was applied as a standard for trehalulose quantification during the sucrose conversion by Klebsiella sp. LX3. The combination of solid-phase extraction and hydrophilic interaction liquid chromatography offers a new avenue for the preparation of sugar isomers from complex natural or fermentation products.