文献类型：期刊文献

英文题名：Dual Flow Reactor Approach for the Chemoenzymatic Production of Chenodeoxycholic Acid, a Precursor to Ursodeoxycholic Acid

作者：Yang, Bing-Yi[1];Liu, Ya-Jing[1];Pan, Jiang[1,2];Zheng, Gao-Wei[1,2];Xu, Jian-He[1,2];Li, Chun-Xiu[1,2]

机构：[1]East China Univ Sci & Technol, Lab Biocatalysis & Synthet Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, Sch Biotechnol, Shanghai 200237, Peoples R China

年份：2026

卷号：74

期号：14

起止页码：11678

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20261720599707);WOS:【SCI-EXPANDED(收录号:WOS:001730262200001)】；

基金：This work was financially supported by the National Key Research and Development Program of China (no. 2024YFA0917800), the National Natural Science Foundation of China (no. 32471495), and the Advanced Materials National Science and Technology Major Project (no. 2025ZD0615001).

语种：英文

外文关键词：enzyme immobilization; enzymecoexpression; chenodeoxycholic acid; Wolff-Kishner-Huangreduction; flow reactor

摘要：Chenodeoxycholic acid, a precursor to ursodeoxycholic acid, can be synthesized from inexpensive cholic acid through a two-step chemoenzymatic route. Here, a dual flow reactor system was developed to intensify this process. First, a packed-bed reactor with coimmobilized enzymes achieved complete conversion of 50 mM cholic acid to 12-oxochenodeoxycholic acid, achieving a space-time yield of 5831 g & centerdot;L-1 & centerdot;d-1, significantly surpassing the batch mode (1632 g & centerdot;L-1 & centerdot;d-1). Subsequently, a PTFE flow reactor facilitated the Wolff-Kishner-Huang reduction, producing chenodeoxycholic acid with a space-time yield of 3629 g & centerdot;L-1 & centerdot;d-1, outperforming the batch mode (1555 g & centerdot;L-1 & centerdot;d-1). This work highlights a safe and efficient platform for chemoenzymatic chenodeoxycholic acid synthesis and provides a generalizable strategy for industrial bile acid production.