文献类型：期刊文献

英文题名：Accelerated myotube formation using bioprinting technology for biosensor applications

作者：Cui, Xiaofeng[1];Gao, Guifang[2];Qiu, Yongjun[3]

机构：[1]Scripps Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA;[2]Wuhan Univ, Inst Virol, Sch Med, Wuhan 430071, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：35

期号：3

起止页码：315

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20130816045864);WOS:【SCI-EXPANDED(收录号:WOS:000314788400003)】；

基金：The authors would like to acknowledge Mr. Jiang Wang for constructive suggestions and technical support. This work was partially funded by National Natural Science Foundation of China (grant no. 2060204).

语种：英文

外文关键词：Bioprinting; Biosensor; C2C12; Muscle contraction; Thermal inkjet

摘要：Muscle-powered, biological, microelectro-mechanical system is promising for actuator and biosensor applications. Functional conjugation between the cells, tissues, and biomolecules to the microdevice is crucial for this application. Bioprinting as an enabling technology possesses the advantages of high throughput, digital control, and highly accurate delivery of various biological factors to the desired locations for numerous applications such as 3D tissue fabrication. We have now evaluated the feasibility of the precise placement of mouse myoblasts onto micro-sized cantilevers. The evenly aligned printed cells fused with each other and formed mature myotubes after only 4 days. In contrast, it took more than 14 days for randomly deposited cells to do so. The printed myotubes were functional and responded to the electrical stimulation synchronously. Furthermore, the integrated Bio-MEMS device responded to the chemical stimulation spontaneously which demonstrated the potential as a functional biosensor. The contractility of the system was recovered quickly after the removal of the chemical stimulation, which indicated the flexibility of this system and the recycling potential.