文献类型：期刊文献

英文题名：Combination of conditioned medium and elicitation enhances taxoid production in bioreactor cultures of Taxus chinensis cells

作者：Wang, ZY; Zhong, JJ

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2002

卷号：12

期号：2

起止页码：93

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000178693200002)】；

语种：英文

外文关键词：bioreactor engineering; combination; conditioned medium; elicitation; plant cell culture; secondary metabolite; taxoid

摘要：Stimulating effects of conditioned medium (CM) addition and its combination with methyl jasmonate (MJA) elicitation on production of physiologically active taxuyunnanine C (TC) by cell cultures of Taxus chinensis were demonstrated in both a shake flask and a 1-1 (working volume) concentric-tube airlift bioreactor. In control cell cultures, average cell growth rates were 0.117 +/- 0.003 per day in the flask and 0.086 +/- 0.001 per day in the airlift bioreactor. With addition of 50% (by volume) CM on day 0, an average growth rate of 0.153 +/- 0.006 per day in flask and 0.125 +/- 0.003 per day in bioreactor was obtained, and the maximum cell density (on day 11) was 15.0 +/- 0.1 g l(-1) in flask and 15.5 +/- 0.8 g l(-1) in bioreactor. TC production and productivity were 43.0 +/- 17.6 mg l(-1) and 1.0 +/- 0.8 mg l(-1) per day in bioreactor control cultures, and a much higher production of 78.9 +/- 20.6 mg l(-1) and productivity of 4.5 +/- 1.2 mg l(-1) per day were obtained in bioreactor cultures with CM addition. Combination of CM addition with MJA (at 100 muM) elicitation enhanced TC productivity in bioreactors by about 30% when compared with the cultures with only MJA elicitation, i.e., increased from 9.2 +/- 0.9 to 11.8 +/- 0.9 mg l(-1) per day. The work is potentially beneficial to large-scale plant cell cultures for production of the bioactive secondary metabolite. (C) 2002 Elsevier Science B.V. All rights reserved.