文献类型：期刊文献

英文题名：A combined feeding strategy for enhancing mycophenolic acid production by fed-batch fermentation in Penicillium brevicompactum

作者：Dong, Yuguo[1];Xu, Rui[1];Wang, Lihua[1];Zhang, Jian[1];Bai, Chaogang[1];Sun, Aiyou[1];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：50

期号：3

起止页码：336

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20150900581181);WOS:【SCI-EXPANDED(收录号:WOS:000352745300002)】；

基金：This study was supported by National Major Science and Technology Projects of China (Project number: 2012ZX09304009).

语种：英文

外文关键词：Mycophenolic acid; Penicillium brevicompactum; Fermentation; Combined feeding strategy

摘要：A combined feeding strategy for enhancing mycophenolic acid production by fed-batch fermentation in Penicillium brevicompactum was investigated. In a 7 L fermenter, the relevant parameters of CN-feeding strategy (F-CN), pH shift strategy (F-pH), and Met-feeding strategy (F-Met) were inspected and analyzed during the synthesis phase of MPA fermentation after 120 h. Compared to the fed-batch fermentation, the three strategies improved the MPA production to 2.23, 1.92, and 2.03 g/L, increased by 29.65, 11.63, and 18.02%, respectively. Based on the above results, a combined strategy (F-CPM) was designed. Under F-CPM strategy, the maximum dry cell weight (DCWmax) reached 45.77 g/L at 240 h, and the maximum MPA production (P-max) reached a higher level of 2.68 g/L, increased by 55.81%. The combined feeding strategy can effectively reduce the impact of substrate inhibition and improve MPA productivity. Thus, our work offered a new idea for industrialization of MPA fermentation with conventional industrial equipment. (C) 2015 Published by Elsevier Ltd.