文献类型：期刊文献

英文题名：Antioxidant and gene expression responses of Eisenia fetida following repeated exposure to BDE209 and Pb in a soil-earthworm system

作者：Hu, Shuangqing[1,2];Zhang, Wei[2];Li, Jing[2];Lin, Kuangfei[2];Ji, Rong[1]

机构：[1]Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Jiangsu, Peoples R China;[2]E China Univ Sci & Technol, Sch Resource & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China

年份：2016

卷号：556

起止页码：163

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：;EI(收录号：20161102099030);WOS:【SCI-EXPANDED(收录号:WOS:000373278700017)】；

基金：This research was supported by projects of the National Natural Science Foundation of China (41371467), and the Shanghai Pujiang Program (15PJD013).

语种：英文

外文关键词：Repeated exposure; BDE209-Pb stress; Gene transcription; Antioxidant enzyme; Eisenia fetida

摘要：This study first adopted repeated treatment model to investigate stress responses in earthworms (Eisenia fetida) following exposure to decabromodiphenyl ether (BDE209) and lead (Pb), which are the mainly co-existed contaminants at e-waste recycling sites. We evaluated the impacts of BDE209-Pb on antioxidative enzyme(superoxide dismutase, SOD; catalase, CAT) activities, malondialdehyde (MDA) contents and transcriptional levels of three target genes (SOD, CAT and Hsp70), and further explored the relationships among these biomarkers. Results demonstrated that almost all the parameters were generally induced and the responses followed certain dose-effect relationships. Compared to the controls, a significant (P < 0.05) up-regulation trend of expression levels of the three genes could be clearly observed after 14 days incubation. Additionally, there existed good correlations between target genes expression levels and antioxidant enzyme activities (R > 0.64). The observations could provide important information of ecotoxicological effects of BDE209-Pb in a soil-earth worm system as well as the mechanism of antioxidant defense. (C) 2016 Elsevier B.V. All rights reserved.