文献类型：期刊文献

英文题名：Development of Dynamic Liquid and Conjugated Electrospun Poly(L-lactide-co-caprolactone)/Collagen Nanoyarns for Regulating Vascular Smooth Muscle Cells Growth

作者：Wu, Tong[1];Zhang, Jialing[2];Wang, Yuanfei[3];Sun, Binbin[1];Guo, Xuran[1];Morsi, Yosry[4];El-Hamshary, Hany[5,6];El-Newehy, Mohamed[5,6];Mo, Xiumei[1,7]

机构：[1]Donghua Univ, Coll Chem Chem Engn & Biotechnol, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 201620, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Med, Shanghai Childrens Med Ctr, Dept Cardiothorac Surg, Shanghai 200127, Peoples R China;[3]East China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]Swinburne Univ Technol, Fac Engn & Ind Sci, Hawthorn, Vic 3122, Australia;[5]King Saud Univ, Dept Chem, Coll Sci, Riyadh 11451, Saudi Arabia;[6]Tanta Univ, Fac Sci, Dept Chem, Tanta 31527, Egypt;[7]Shandong Int Biotechnol Pk Dev Co Ltd, Yantai 264000, Peoples R China

年份：2017

卷号：13

期号：3

起止页码：303

外文期刊名：JOURNAL OF BIOMEDICAL NANOTECHNOLOGY

收录：;EI(收录号：20171303492956);WOS:【SCI-EXPANDED(收录号:WOS:000397029400006)】；

基金：This research was supported by National Major Research Program of China (2016YFC1100202), National Natural Science Foundation of China (31470941, 31271035), Science and Technology Commission of Shanghai Municipality (15JC1490100, 15441905100), Yantai Double Handred Talent Plan, Ph.D. Programs Foundation of Ministry of Education of China (20130075110005) and light of textile project (J201404), The authors extend their appreciation to the International Scientific Partnership Program ISPP at King Saud University for funding this research work through ISPP# 0049.

语种：英文

外文关键词：Electrospinning; Nanoyarns; P(LLA-CL); Collagen; Smooth Muscle Cells

摘要：Simulating the modeling of smooth muscle layer in the vascular structure makes a great difference for vascular tissue regeneration. A functional tissue engineered vascular media shall promote the aligned organization and three-dimensional penetration of smooth muscle cells (SMCs) into the scaffold. To this aim, dynamic liquid and conjugated nanoyarns based on poly(L-lactide-co-caprolactone) (P(LLA-CL)) and collagen (COL) with a weight ratio at 3: 1 were fabricated by electrospinning methods, with random and aligned nanofibers as control groups. The Fourier transform infrared spectroscopy and X-ray diffraction analyses confirmed the preservation of P(LLA-CL)/COL components and structure. Scanning electron microscope (SEM) results indicated a significant increase of yarn diameters at 19.27 +/- 6.16 mu m (dynamic liquid) and 10.24 +/- 3.09 mu m (conjugated), and both of the nanoyarns had improved mechanical tensile properties than the random nanofibers. Compared with random and aligned nanofibers, the nanoyarns presented significant higher porosity and larger pore diameter, leading to a decrease of water contact angle and a promotion of SMCs proliferation and migration. Better SMCs orientation was observed on the conjugated nanoyarns, while superior SMCs penetration was achieved on the dynamic liquid nanoyarns, owing to the differences in yarns microstructure. Herein, this study demonstrated that the aligned and porous P(LLA-CL)/COL nanoyarns fabricated by dynamic liquid and conjugated electrospinning were beneficial to regulating vascular SMCs outgrowth, which had important implications for functional reconstruction of vascular media.