文献类型：期刊文献

英文题名：Fipronil induces apoptosis through caspase-dependent mitochondrial pathways in Drosophila S2 cells

作者：Zhang, Baoyan[1];Xu, Zhiping[1];Zhang, Yixi[1,2];Shao, Xusheng[1];Xu, Xiaoyong[1];Cheng, Jiaogao[1];Li, Zhong[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Minist Educ, Nanjing 210095, Jiangsu, Peoples R China

年份：2015

卷号：119

起止页码：81

外文期刊名：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000353367000012)】；

基金：This work was financial supported by National High Technology Research Development Program of China (863 Program, 2011AA10A207), National Natural Science Foundation of China (21372079, 21472046, 21172070), General Financial Grant from the China Postdoctoral Science Foundation (2012M520853) and the Fundamental Research Funds for the Central Universities (WY1214030).

语种：英文

外文关键词：Fipronil; Drosophila S2 cells; Apoptosis; Mitochondrion; Caspase; Cytochrome c

摘要：Fipronil is the first phenylpyrazole insecticide widely used in controlling pests, including pyrethroid, organophosphate and carbamate insecticides. It is generally accepted that fipronil elicits neurotoxicity via interactions with GABA and glutamate receptors, although alternative mechanisms have recently been proposed. This study evaluates the genotoxicity of fipronil and its likely mode of action in Drosophila S2 cells, as an in vitro model. Fipronil administrated the concentration- and time-dependent S2 cell proliferation. Intracellular biochemical assays showed that fipronil-induced S2 cell apoptosis coincided with a decrease in the mitochondrial membrane potential and an increase reactive oxygen species generation, a significant decrease of Bcl-2 and DIAP1, and a marked augmentation of Cyt c and caspase-3. Because caspase-3 is the major executioner caspase downstream of caspase-9 in Drosophila, enzyme activity assays were used to determine the activities of caspase-3 and caspase-9. Our results indicated that fipronil effectively induced apoptosis in Drosophila S2 cells through caspase-dependent mitochondrial pathways. (C) 2015 Elsevier Inc. All rights reserved.