文献类型：期刊文献

英文题名：Bioactive borate glass scaffold for bone tissue engineering

作者：Liang, Wen[1];Rahaman, Mohamed N.[2,3];Day, Delbert E.[2,3];Marion, Nicholas W.[4];Riley, Gwendolen C.[4];Mao, Jeremy J.[4]

机构：[1]E China Univ Sci & Technol, Inst Biomat, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China;[2]Univ Missouri, Dept Ceram Engn, Rolla, MO 65409 USA;[3]Univ Missouri, Mat Res Ctr, Rolla, MO 65409 USA;[4]Univ Illinois, Dept Bioengn, Chicago, IL 60607 USA

年份：2008

卷号：354

期号：15-16

起止页码：1690

外文期刊名：JOURNAL OF NON-CRYSTALLINE SOLIDS

收录：;EI(收录号：20080811109351);WOS:【SCI-EXPANDED(收录号:WOS:000254353300016)】；

语种：英文

外文关键词：bioglass; scanning electron microscopy; porosity; borates

摘要：Borate glass particles and microspheres with size distributions in the range of approximately 100-400 mu m, were loosely compacted and sintered for 10 min at 600 degrees C to form a porous, three-dimensional construct (porosity 25-40%). Conversion of the borate glass to hydroxyapatite was investigated by soaking the constructs in a solution of K2HPO4 (0.25 M) at 37 degrees C and with a pH value of 9.0, and measuring the weight loss of the constructs as a function of time. Almost full conversion of the borate glass to hydroxyapatite was achieved in less than 6 days. X-ray diffraction revealed an initially amorphous product that subsequently crystallized to hydroxyapatite. The biocompatibility of the porous constructs was investigated by in vitro cell culture with human mesenchymal stem cells derived from bone marrow (bMSC) and human mesenchymal stem cell derived osteoblasts (MSC-Ob). The cells adhered to the scaffolds and the MSC-Obs produced alkaline phosphatase which is an indication of osteogenic differentiation. The data suggest strong bioactive characteristics for the borate glass constructs and the potential use of the constructs as scaffolds for tissue engineering of bone. (c) 2007 Elsevier B.V. All rights reserved.