文献类型：期刊文献

英文题名：Conditional ablation of protein tyrosine phosphatase receptor U in midbrain dopaminergic neurons results in reduced neuronal size

作者：Liu, Jiwei[1];Yang, Li[2];Li, Han[1];Cai, Yichuan[3];Feng, Jiali[3];Hu, Zelan[3]

机构：[1]Shanghai Jiao Tong Univ, Sch Med, Shanghai Mental Hlth Ctr, Shanghai 200030, Peoples R China;[2]Naval Medical Univ, Affiliated Hosp 2, Dept Anesthesiol, Shanghai 200030, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, 130 Mei Long Rd, Shanghai 200237, Peoples R China

年份：2022

卷号：124

外文期刊名：JOURNAL OF CHEMICAL NEUROANATOMY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000862591100004)】；

基金：We are grateful for the financial support from the National Natural Science Foundation of China [Grant numbers: 31300904 and 81772689] . This work was sponsored by Shanghai Sailing Program [Grant number: 20YF1442200] and Natural Science Foundation of Shanghai [Grant number: 21ZR1455200] .

语种：英文

外文关键词：protein tyrosine phosphatase receptor U  reduced neuronal size; mDA neurons; orphan nuclear receptor; tyrosine hydroxylase

摘要：Protein tyrosine phosphatase receptor U (PTPRU) is involved in midbrain patterning during early stages of development and is continuously expressed in the adult midbrain areas where dopaminergic neurons reside in. However, whether PTPRU is also involved in the maintenance and survival of midbrain dopaminergic (mDA) neurons during the late stages of development or in the adult midbrain remains largely unknown. In the present study, Ptpru was ablated by crossing a floxed Ptpru mouse strain with tyrosine hydroxylase (TH)-Cre mice that express Cre recombinase in postmitotic mDA neurons. Conditional ablation of Ptpru in postmitotic mDA neurons resulted in a reduction of somatic and nuclear size in adulthood. However, TH-immunoreactivity of Ptpru-ablated mDA neurons and their projections to the striatum appeared undisturbed. We also investigated the maintenance of several mDA neuronal markers following Ptpru ablation and found no significant changes. Taken together, these findings suggest that PTPRU is involved in regulating the neuronal size of mDA neurons and provided mechanistic insights into the development and maintenance of mDA neurons.