文献类型：期刊文献

英文题名：Increasing the Productivity of TNFR-Fc in GS-CHO Cells at Reduced Culture Temperatures

作者：Kou, Tian-Ci[1];Fan, Li[1];Zhou, Yan[1];Ye, Zhao-Yang[1];Zhao, Liang[1];Tan, Wen-Song[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2011

卷号：16

期号：1

起止页码：136

外文期刊名：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

收录：;EI(收录号：20120514728526);WOS:【SCI-EXPANDED(收录号:WOS:000287959500017)】；

语种：英文

外文关键词：cell growth arrest; GS-CHO cells; low temperature; TNFR-Fc; transcriptional level

摘要：In an effort to improve TNFR-Fc production in GS-CHO cells, batch cultures were performed to investigate the effects of low culture temperature on TNFR-Fc production. It was observed that low culture temperatures resulted in cell cycle arrest in the G1 phase, led to suppressed cell growth, and prolonged the culture period. Although the highest TNFR-Fc concentration was achieved with a culture temperature of 32 degrees C at 247.4 mg/L, the maximum q(TNFR-Fc) of 15.7 pg/cells/day was achieved at 30 degrees C. Because the inhibition effect on cell growth at 30 degrees C compromised its beneficial effects specifically to TNFR-Fc productivity, TNFR-Fc concentration at this temperature was not significantly increased. Furthermore, the increase in productivity of specific TNFR-Fc at low culture temperatures was also found to be due to an increase in the transcriptional level of the TNFR-Fc gene, determined by RT-PCR analysis. In addition, low culture temperatures had no significant effect on the degree of sialylation of TNFR-Fc. Taken together; a biphasic cultivation process developed in a fed-batch mode with a low temperature-production phase enhanced TNFR-Fc production by GS-CHO cells and therefore offers major potential for bioprocess optimization.